Toxicological evaluation of ionic liquid in a biological functional tissue construct model based on nano-hydroxyapatite/chitosan/gelatin hybrid scaffolds

doi:10.1016/j.ijbiomac.2020.04.267

	Toxicological evaluation of ionic liquid in a biological functional tissue construct model based on nano-hydroxyapatite/chitosan/gelatin hybrid scaffolds
	Zhu, Jingjing 3; Kong, Qian 3; Zheng, Shuangshuang 1; Wang, Yiwei 2; Jiao, Zeren 4; Nie, Yi 1,5; Liu, Tianqing 3; Song, Kedong 3
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2020-09-01
卷号	158 页码:800-810
关键词	Ionic liquid Cytotoxicity Three-dimensional tissue constructs
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2020.04.267
英文摘要	The application of ionic liquid is attracting more attentions as green replacement for volatile organic solvents. However, the toxic effects and risks of ionic liquid in different biological systems for human health and environ-ment are poorly evaluated. Among all ionic liquids, 1-ethyl-3-methylimidazolium diethylphosphate ([Emim] DEP-type) ionic liquid is still at the early phase of development, and its toxicity remains unclear. In this study, we fabricated a 3D biological functional tissue construct model based on nano-hydroxyapatite, chitosan and gel-atin hybrid scaffold and evaluated its toxic effects of [Emim]DEP-type ionic liquid. As a control group, the exam-ination of ionic liquid's toxic effects on the pre-osteoblast cell line (MC3T3-E1) was detected in 2D cultures. The MTT assay showed that [Emim]DEP-type ionic liquid inhibited the proliferation of cells on both 2D cultures and 3D tissue constructs. This effect was correlated with culturing time and concentration, while the IC50 on 3D scaf-folds (12,566, 9015, 7896 mu g/mL, at 24 h, 48 h and 72 h, respectively) was found significantly higher compared to 2D cultures (3959, 2226,1884 mu g/mL). Flow cytometry analysis and scanning electron microscope demonstrated that when [Emim]DEP-type ionic liquid acted on MC3T3-E1 cells for 48 h, the shape of 2D cells shrank, together with decreased surface adhesion. (C) 2020 Published by Elsevier B.V.
资助项目	National Natural Science Foundation of China[31670978/21676041] ; Fok Ying Tung Education Foundation[132027] ; State Key Laboratory of Fine Chemicals[KF1111] ; Natural Science Foundation of Liaoning[20180510028] ; Fundamental Research Funds for the Central Universities[DUT20ZD206]
WOS关键词	FISH CELL-LINES ; 1-OCTYL-3-METHYLIMIDAZOLIUM BROMIDE ; MICROWAVE SYNTHESIS ; CYTOTOXICITY ; TOXICITY ; ACID ; ESTERIFICATION ; ECOTOXICOLOGY ; EXTRACTION ; CHLORIDE
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000564484800007
资助机构	National Natural Science Foundation of China ; Fok Ying Tung Education Foundation ; State Key Laboratory of Fine Chemicals ; Natural Science Foundation of Liaoning ; Fundamental Research Funds for the Central Universities
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/41956]
专题	中国科学院过程工程研究所
通讯作者	Nie, Yi; Liu, Tianqing; Song, Kedong
作者单位	1.Zhengzhou Inst Emerging Ind Technol, Zhengzhou 450000, Peoples R China 2.Univ Sydney, ANZAC Res Inst, Burns Res Grp, Concord, NSW 2139, Australia 3.Dalian Univ Technol, Dalian R&D Ctr Stem Cell & Tissue Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China 4.Texas A&M Univ, Artie McFerrin Dept Chem Engn, College Stn, TX 77843 USA 5.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Jingjing,Kong, Qian,Zheng, Shuangshuang,et al. Toxicological evaluation of ionic liquid in a biological functional tissue construct model based on nano-hydroxyapatite/chitosan/gelatin hybrid scaffolds[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,158:800-810.
APA	Zhu, Jingjing.,Kong, Qian.,Zheng, Shuangshuang.,Wang, Yiwei.,Jiao, Zeren.,...&Song, Kedong.(2020).Toxicological evaluation of ionic liquid in a biological functional tissue construct model based on nano-hydroxyapatite/chitosan/gelatin hybrid scaffolds.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,158,800-810.
MLA	Zhu, Jingjing,et al."Toxicological evaluation of ionic liquid in a biological functional tissue construct model based on nano-hydroxyapatite/chitosan/gelatin hybrid scaffolds".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 158(2020):800-810.