Efficient ex-situ biosynthesis of vitamin B-12 by Propionibacterium freudenreichii using membrane separation coupling technology

doi:10.1016/j.bej.2020.107688

	Efficient ex-situ biosynthesis of vitamin B-12 by Propionibacterium freudenreichii using membrane separation coupling technology
	Wang, Ziqiang; Xu, Guoxia; Du, Wei; Zhang, Yuhan; Wang, Yunshan; Su, Zhiguo
刊名	BIOCHEMICAL ENGINEERING JOURNAL
	2020-09-15
卷号	161 页码:7
关键词	Propionibacterium freudenreichii Vitamin B-12 Adenosylcobinamide Ex situ biosynthesis Membrane separation coupling fermentation
ISSN号	1369-703X
DOI	10.1016/j.bej.2020.107688
英文摘要	Propionibacterium freudenreichii has been successfully applied to industrial anaerobic production of vitamin B-12. In the traditional fed-batch fermentation process, 5,6-dimethylbenzimidazole (DMB) is used as a precursor. However, DMB can inhibit the synthesis of vitamin B-12 metabolic intermediates, which is not conducive to semicontinuous fermentation of P. freudenreichii. To improve the fermentation efficiency, vitamin B-12 ex-situ biosynthesis was proposed and a membrane separation coupling fermentation technology was designed with adenosylcobinamide (Ado-cbi) as the indicator signal. The results showed that P. freudenreichii cells should be partly separated online at 84 h with 0.22-mu m spiral membrane. The fourfold concentrated suspension was suitable for ex-situ biosynthesis of vitamin B-12 with the addition of 3.60 mg L-1 DMB at 30 degrees C and pH 7.0. In the semi-continuous coupling fermentation process, vitamin B-12 concentration reached 56.76 +/- 3.86 mg L-1 , an improvement of 161.6 %, while reutilizing fermentation wastewater and improving the anaerobic fermentation efficiency of P. freudenreichii.
资助项目	National Natural Science Foundation of China[21506227]
WOS关键词	PROPIONIC-ACID ; COBALAMIN VITAMIN-B-12 ; MICROBIAL-PRODUCTION ; CONTROL STRATEGY ; FERMENTATION ; REMOVAL ; ENZYME
WOS研究方向	Biotechnology & Applied Microbiology ; Engineering
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000552131300037
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/41457]
专题	中国科学院过程工程研究所
通讯作者	Wang, Yunshan
作者单位	Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ziqiang,Xu, Guoxia,Du, Wei,et al. Efficient ex-situ biosynthesis of vitamin B-12 by Propionibacterium freudenreichii using membrane separation coupling technology[J]. BIOCHEMICAL ENGINEERING JOURNAL,2020,161:7.
APA	Wang, Ziqiang,Xu, Guoxia,Du, Wei,Zhang, Yuhan,Wang, Yunshan,&Su, Zhiguo.(2020).Efficient ex-situ biosynthesis of vitamin B-12 by Propionibacterium freudenreichii using membrane separation coupling technology.BIOCHEMICAL ENGINEERING JOURNAL,161,7.
MLA	Wang, Ziqiang,et al."Efficient ex-situ biosynthesis of vitamin B-12 by Propionibacterium freudenreichii using membrane separation coupling technology".BIOCHEMICAL ENGINEERING JOURNAL 161(2020):7.