Acceptor engineering of small-molecule fluorophores for NIR-II fluorescence and photoacoustic imaging

doi:10.1039/d1tb02282b

	Acceptor engineering of small-molecule fluorophores for NIR-II fluorescence and photoacoustic imaging
	Li, Yaxi 1; Zhou, Hongli 2; Bi, Renzhe 3; Li, Xiuting 3; Zha, Menglei 1; Yang, Yanqing 2; Ni, Jen-Shyang 1; Liew, Weng Heng 4; Olivo, Malini 3; Yao, Kui 4
刊名	JOURNAL OF MATERIALS CHEMISTRY B
	2021-12-15
卷号	9 期号:48 页码:9951-9960
ISSN号	2050-750X
DOI	10.1039/d1tb02282b
通讯作者	Liu, Jie(iamjieliu@njtech.edu.cn) ; Chen, Hao(haoc@simm.ac.cn) ; Li, Kai(lik@sustech.edu.cn)
英文摘要	Fluorescence imaging in the second near-infrared window (NIR-II) has been an emerging technique in diverse in vivo applications with high sensitivity/resolution and deep tissue penetration. To date, the design principle of the reported NIR-II organic fluorophores has heavily relied on benzo[1,2-c:4,5-c ']bis([1,2,5]thiadiazole) (BBTD) as a strong electron acceptor. Here, we report the rational design and synthesis of a NIR-II fluorescent molecule with the rarely used [1,2,5]thiadiazolo[3,4-f]benzotriazole (TBZ) core to replace BBTD as the electron acceptor. Thanks to the weaker electron deficiency of the TBZ core than BBTD, the newly yielded NIR-II molecule (BTB) based nanoparticles have a higher mass extinction coefficient and quantum yield in water. In contrast, the nanoparticle suspension of its counterpart with BBTD as the core is nearly nonemissive. The NIR-II BTB nanoparticles allow video-rate fluorescence imaging for vasculature imaging in ears, hindlimbs, and the brain of the mouse. Additionally, its large absorptivity in the NIR-I region also promotes bioimaging using photoacoustic microscopy (PAM) and tomography (PAT). Upon surface conjugation with the Arg-Gly-Asp (RGD) peptide, the functionalized nanoparticles ensured targeted detection of integrin-overexpressed tumors through both imaging modalities in two- and three-dimensional views. Thus, our approach to engineering acceptors of organic fluorophores offers a promising molecular design strategy to afford new NIR-II fluorophores for versatile biomedical imaging applications.
资助项目	Ministry of Science and Technology of China[2020YFA0908900] ; National Natural Science Foundation of China[31870991] ; National Natural Science Foundation of China[82071976] ; NanjingTech Startup Grant[38274017115] ; Guangdong Innovative and Entrepreneurial Research Team Program[2019ZT08Y191] ; Shenzhen Key Laboratory of Smart Healthcare Engineering[ZDSYS20200811144003009] ; Science and Technology Innovation Committee of Shenzhen Municipality[JCYJ20190809154011696] ; Science and Technology Innovation Committee of Shenzhen Municipality[JCYJ20200109141222892] ; Shanghai Municipal Science and Technology Major Project ; Shanghai Pujiang Program[19PJ1411100] ; National Research Foundation Competitive Research Programme of Singapore[NRF-CRP15-2015-04] ; National Research Foundation Competitive Research Programme of Singapore[IMRE/16-9P1122]
WOS关键词	WINDOW
WOS研究方向	Materials Science
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000725115100001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/299055]
专题	中国科学院上海药物研究所
通讯作者	Liu, Jie; Chen, Hao; Li, Kai
作者单位	1.Southern Univ Sci & Technol, Shenzhen Key Lab Smart Healthcare Engn, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China 2.Nanjing Tech Univ Nanjing Tech, Key Lab Flexible Elect KLOFE, Inst Adv Mat IAM, 30 South Puzhu Rd, Nanjing 211800, Peoples R China 3.A STAR Agency Sci Technol & Res, Inst Bioengn & Bioimaging IBB, 11 Biopolis Way, Singapore, Singapore 4.A STAR Agency Sci Technol & Res, Inst Mat Res & Engn, 2 Fusionopolis Way, Innovis 138634, Singapore 5.Chinese Acad Sci, Shanghai Inst Mat Med, Mol Imaging Ctr, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Li, Yaxi,Zhou, Hongli,Bi, Renzhe,et al. Acceptor engineering of small-molecule fluorophores for NIR-II fluorescence and photoacoustic imaging[J]. JOURNAL OF MATERIALS CHEMISTRY B,2021,9(48):9951-9960.
APA	Li, Yaxi.,Zhou, Hongli.,Bi, Renzhe.,Li, Xiuting.,Zha, Menglei.,...&Li, Kai.(2021).Acceptor engineering of small-molecule fluorophores for NIR-II fluorescence and photoacoustic imaging.JOURNAL OF MATERIALS CHEMISTRY B,9(48),9951-9960.
MLA	Li, Yaxi,et al."Acceptor engineering of small-molecule fluorophores for NIR-II fluorescence and photoacoustic imaging".JOURNAL OF MATERIALS CHEMISTRY B 9.48(2021):9951-9960.