Development of sensitive genotype-specific quantitative polymerase chain reaction methods for detection of Phaeocystis globosa in the South China Sea

doi:10.1002/lom3.10476

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Development of sensitive genotype-specific quantitative polymerase chain reaction methods for detection of Phaeocystis globosa in the South China Sea
	Niu, Zhuang 1,2,3,4; Liu, Chao 1,2,3,4; Zhang, Qing-Chun 1,2,4; Wang, Jin-Xiu 1,2,3,4; Kong, Fan-Zhou 1,2,4; Hu, Xiao-Kun 1,2,3,4; Yu, Ren-Cheng 1,2,3,4
刊名	LIMNOLOGY AND OCEANOGRAPHY-METHODS
	2021-12-27
页码	15
ISSN号	1541-5856
DOI	10.1002/lom3.10476
通讯作者	Zhang, Qing-Chun(qczhang@qdio.ac.cn)
英文摘要	Since 1997, dense blooms of the alga Phaeocystis globosa have occurred in the South China Sea (SCS), and previous studies have revealed the co-existence in this sea of two types of P. globosa cell characterized by different marker pigment profiles. However, owing to a lack of quantitative methods for the detection of different types of P. globosa cells, the dynamics of P. globosa blooms in the SCS are still poorly understood. In this study, on the basis of phylogenetic analysis of the mitochondrial atp8 (Mit atp8) gene and pigment profiles, the P. globosa strains from Pacific and Atlantic coastal waters were divided into four genotypes, of which Type I and Type IV strains co-exist in the SCS. Furthermore, two genotype-specific quantitative polymerase chain reaction (qPCR) methods were developed targeting the Mit atp8 gene for quantitative determinations of the densities of Type I and Type IV P. globosa cells. Both qPCR methods were reproducible, highly sensitive, and specific with a wide range of detection (from 30 to 1 x 10(8) cells L-1) for target P. globosa genotypes in the field, thereby facilitating the detection of low cell densities prior to bloom development, as well as high cell densities during bloom periods. Finally, the developed qPCR assays were successfully applied to determine the distribution patterns of Type I and Type IV P. globosa in the Beibu Gulf, a region of the SCS characterized by a high frequency of P. globosa blooms.
资助项目	National Key R&D Program of China[2017YFC1404304] ; National Natural Science Foundation of China[42076140] ; National Natural Science Foundation of China[U20A20104] ; National Natural Science Foundation of China[41776127] ; S&T Basic Resources Investigation Program of China[2018YF100206]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
语种	英语
出版者	WILEY
WOS记录号	WOS:000734508400001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/177593]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Zhang, Qing-Chun
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Niu, Zhuang,Liu, Chao,Zhang, Qing-Chun,et al. Development of sensitive genotype-specific quantitative polymerase chain reaction methods for detection of Phaeocystis globosa in the South China Sea[J]. LIMNOLOGY AND OCEANOGRAPHY-METHODS,2021:15.
APA	Niu, Zhuang.,Liu, Chao.,Zhang, Qing-Chun.,Wang, Jin-Xiu.,Kong, Fan-Zhou.,...&Yu, Ren-Cheng.(2021).Development of sensitive genotype-specific quantitative polymerase chain reaction methods for detection of Phaeocystis globosa in the South China Sea.LIMNOLOGY AND OCEANOGRAPHY-METHODS,15.
MLA	Niu, Zhuang,et al."Development of sensitive genotype-specific quantitative polymerase chain reaction methods for detection of Phaeocystis globosa in the South China Sea".LIMNOLOGY AND OCEANOGRAPHY-METHODS (2021):15.