Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals

doi:10.1038/s41467-021-23216-7

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	Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals
	Xu, Fei 1,2; Marletaz, Ferdinand 1,3,5; Gavriouchkina, Daria 4,5; Liu, Xiao 6; Sauka-Spengler, Tatjana 4; Zhang, Guofan 2,7; Holland, Peter W. H.1
刊名	NATURE COMMUNICATIONS
	2021-05-25
卷号	12 期号:1 页码:11
ISSN号	2041-1723
DOI	10.1038/s41467-021-23216-7
通讯作者	Xu, Fei(xufei@qdio.ac.cn)
英文摘要	Hox and ParaHox genes encode transcription factors with similar expression patterns in divergent animals. The Pdx (Xlox) homeobox gene, for example, is expressed in a sharp spatial domain in the endodermal cell layer of the gut in chordates, echinoderms, annelids and molluscs. The significance of comparable gene expression patterns is unclear because it is not known if downstream transcriptional targets are also conserved. Here, we report evidence indicating that a classic transcriptional target of Pdx1 in vertebrates, the insulin gene, is a likely direct target of Pdx in Pacific oyster adults. We show that one insulin-related gene, cgILP, is co-expressed with cgPdx in oyster digestive tissue. Transcriptomic comparison suggests that this tissue plays a similar role to the vertebrate pancreas. Using ATAC-seq and ChIP, we identify an upstream regulatory element of the cgILP gene which shows binding interaction with cgPdx protein in oyster hepatopancreas and demonstrate, using a cell culture assay, that the oyster Pdx can act as a transcriptional activator through this site, possibly in synergy with NeuroD. These data argue that a classic homeodomain-target gene interaction dates back to the origin of Bilateria. In vertebrates insulin is a direct transcriptional target of Pdx: the same is true in Pacific oysters and the authors show insulin-related gene, cgILP, is co-expressed with cgPdx in oyster digestive tissue, showing this gene interaction dates back to the origin of Bilateria.
资助项目	Wellcome Trust[090532/Z/09/Z] ; National Natural Science Foundation of China[41776152] ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Science[COMS2019Q11] ; China Scholarship Council (CSC) ; European Research Council under the European Union[268513] ; Royal Society International Exchanges grant ; QR GCRF award
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE RESEARCH
WOS记录号	WOS:000658773600012
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/176918]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Xu, Fei
作者单位	1.Univ Oxford, Dept Zool, Oxford, England 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.UCL, Ctr Lifes Origins & Evolut, Dept Genet Evolut & Environm, London, England 4.Univ Oxford, Weatherall Inst Mol Med, Radcliffe Dept Med, Oxford, England 5.Okinawa Inst Sci & Technol Grad Univ, Mol Genet Unit, Onna, Okinawa, Japan 6.Zhejiang Ocean Univ, Fishery Coll, Key Lab Marine Fishery Equipment & Technol Zhejia, Zhoushan, Peoples R China 7.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Xu, Fei,Marletaz, Ferdinand,Gavriouchkina, Daria,et al. Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals[J]. NATURE COMMUNICATIONS,2021,12(1):11.
APA	Xu, Fei.,Marletaz, Ferdinand.,Gavriouchkina, Daria.,Liu, Xiao.,Sauka-Spengler, Tatjana.,...&Holland, Peter W. H..(2021).Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals.NATURE COMMUNICATIONS,12(1),11.
MLA	Xu, Fei,et al."Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals".NATURE COMMUNICATIONS 12.1(2021):11.