Chloroplast Genetic Engineering of a Unicellular Green AlgaHaematococcus pluvialiswith Expression of an Antimicrobial Peptide

doi:10.1007/s10126-020-09978-z

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Chloroplast Genetic Engineering of a Unicellular Green AlgaHaematococcus pluvialiswith Expression of an Antimicrobial Peptide
	Wang, Kang 1; Gao, Zhengquan 1; Meng, Chunxiao 1; Cui, Yulin 2,3; Wang, Yinchu 2,3; Qin, Song 2,3; Liu, Tianzhong 4,5
刊名	MARINE BIOTECHNOLOGY
	2020-08
卷号	22 期号:4 页码:572-580
关键词	Haematococcus pluvialis Chloroplast transformation Antimicrobial peptide Bar Biolistic method
ISSN号	1436-2228
DOI	10.1007/s10126-020-09978-z
英文摘要	The purpose of this study was to express an antimicrobial peptide in the chloroplast to further develop the plastid engineering ofH. pluvialis. Homologous targeting of the 16S-trnI/trnA-23S region and four endogenous regulatory elements, including thepsbApromoter,rbcLpromoter,rbcLterminator, andpsbAterminator inH. pluvialis, were performed to construct a chloroplast transformation vector forH. pluvialis. The expression of codon-optimized antimicrobial peptide piscidin-4 gene (ant1) and selection marker gene (bar, biolaphos resistance gene) in the chloroplast ofH. pluvialiswas controlled by therbcLpromoter andpsbApromoter, respectively. Upon biolistic transformation and selection with phosphinothricin, integration and expression ofant1in the chloroplast genome were detected using polymerase chain reaction (PCR), southern blotting, and western blotting. Using this method, we successfully expressed antimicrobial peptide piscidin-4 inH. pluvialis. Hence, our results showedH. pluvialispromises as a platform for expressing recombinant proteins for biotechnological applications, which will further contribute to promoting genetic engineering improvement of this strain.
WOS关键词	HAEMATOCOCCUS-PLUVIALIS ; ASTAXANTHIN ACCUMULATION ; ALGA HAEMATOCOCCUS ; MICROALGAE ; GROWTH ; PATHWAYS ; PROTEINS ; GENOMES
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种	英语
WOS记录号	WOS:000539935200001
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41876188, 31972815] ; Major Basic Research Program of Shandong Province Natural Science Foundation [ZR2018ZB0210, ZR2019ZD17] ; Project of Innovation & Development of Marine Economy [HHCL201803] ; Key Research and Development Program of Shandong Province (Food for Special Medical Purpose) [2018YYSP016] ; Key Research and Development Program of Shandong Province [2017CXGC0309] ; Project of Shandong Province Higher Educational Science and Technology Program [J17KA132]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/30482]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Shandong, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China; 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China; 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Microalgal Biotechnol Grp, CAS Key Lab Biofuels, Qingdao 266101, Peoples R China; 5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Lab Energy Genet, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Wang, Kang,Gao, Zhengquan,Meng, Chunxiao,et al. Chloroplast Genetic Engineering of a Unicellular Green AlgaHaematococcus pluvialiswith Expression of an Antimicrobial Peptide[J]. MARINE BIOTECHNOLOGY,2020,22(4):572-580.
APA	Wang, Kang.,Gao, Zhengquan.,Meng, Chunxiao.,Cui, Yulin.,Wang, Yinchu.,...&Liu, Tianzhong.(2020).Chloroplast Genetic Engineering of a Unicellular Green AlgaHaematococcus pluvialiswith Expression of an Antimicrobial Peptide.MARINE BIOTECHNOLOGY,22(4),572-580.
MLA	Wang, Kang,et al."Chloroplast Genetic Engineering of a Unicellular Green AlgaHaematococcus pluvialiswith Expression of an Antimicrobial Peptide".MARINE BIOTECHNOLOGY 22.4(2020):572-580.