Preparation and application of novel microspheres possessing autofluorescent properties

CORC > 过程工程研究所 > 中国科学院过程工程研究所 > 研究所（批量导入）

	Preparation and application of novel microspheres possessing autofluorescent properties
	Wei, Wei ; Wang, Lian-Yan ; Yuan, Lan ; Wei, Qiang ; Yang, Xiao-Da ; Su, Zhi-Guo ; Ma, Guang-Hui
刊名	ADVANCED FUNCTIONAL MATERIALS
	2007-11-05
卷号	17 期号:16 页码:3153-3158
关键词	fluorescent latex microspheres blood-flow distribution nanoparticles nanospheres
ISSN号	1616-301X
其他题名	Adv. Funct. Mater.
中文摘要	Fluorescent microspheres are widely used as biological tracers. In this study, uniformly sized chitosan microspheres crosslinked with glutaraldehyde (CG microspheres) and formaldehyde (CF microspheres) are successfully prepared by the Shirasu Porous Glass (SPG) membrane emulsification technique. Selectively reduced CG microspheres (SRCG microspheres) are obtained by NaBH4 reduction. These chitosan microspheres are found to exhibit fluorescent properties without conjugation to any fluorescent agent. The fluorescence color varies with different crosslinkers and can be modulated by further chemical reduction, whereas the fluorescence intensity can be controlled by tuning the particle size and degree of crosslinking. The auto-fluorescence of the microspheres is applied to study the phagocytosis of HepG2 cells using the microspheres as novel tracers. Quantitative and qualitative evaluations show that these chitosan microspheres serve as bright, inert, durable, and extremely photostable tracers.
英文摘要	Fluorescent microspheres are widely used as biological tracers. In this study, uniformly sized chitosan microspheres crosslinked with glutaraldehyde (CG microspheres) and formaldehyde (CF microspheres) are successfully prepared by the Shirasu Porous Glass (SPG) membrane emulsification technique. Selectively reduced CG microspheres (SRCG microspheres) are obtained by NaBH4 reduction. These chitosan microspheres are found to exhibit fluorescent properties without conjugation to any fluorescent agent. The fluorescence color varies with different crosslinkers and can be modulated by further chemical reduction, whereas the fluorescence intensity can be controlled by tuning the particle size and degree of crosslinking. The auto-fluorescence of the microspheres is applied to study the phagocytosis of HepG2 cells using the microspheres as novel tracers. Quantitative and qualitative evaluations show that these chitosan microspheres serve as bright, inert, durable, and extremely photostable tracers.
WOS标题词	Science & Technology ; Physical Sciences ; Technology
类目[WOS]	Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
研究领域[WOS]	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
关键词[WOS]	FLUORESCENT LATEX MICROSPHERES ; BLOOD-FLOW DISTRIBUTION ; NANOPARTICLES ; NANOSPHERES
收录类别	SCI
原文出处	://WOS:000250989600017
语种	英语
WOS记录号	WOS:000250989600017
公开日期	2013-10-15
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3474]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Peking Univ, Hlth Sci Ctr, Med & Hlth Analyt Ctr, Beijing 100083, Peoples R China 4.Peking Univ, Hlth Sci Ctr, State Key Labs Nat & Biomimet Drugs, Dept Biol Chem, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Wei, Wei,Wang, Lian-Yan,Yuan, Lan,et al. Preparation and application of novel microspheres possessing autofluorescent properties[J]. ADVANCED FUNCTIONAL MATERIALS,2007,17(16):3153-3158.
APA	Wei, Wei.,Wang, Lian-Yan.,Yuan, Lan.,Wei, Qiang.,Yang, Xiao-Da.,...&Ma, Guang-Hui.(2007).Preparation and application of novel microspheres possessing autofluorescent properties.ADVANCED FUNCTIONAL MATERIALS,17(16),3153-3158.
MLA	Wei, Wei,et al."Preparation and application of novel microspheres possessing autofluorescent properties".ADVANCED FUNCTIONAL MATERIALS 17.16(2007):3153-3158.