Effect of Wnt signaling on the zebrafish embryonic developmental toxicity induced by lead exposure.

	Effect of Wnt signaling on the zebrafish embryonic developmental toxicity induced by lead exposure.
	Yan Xiaotao; Gui Fang; Zhang Jinhua; Si Jing; Zhang Hong
刊名	Chinese Journal of Applied and Environmental Biology
	2019
卷号	25 期号:3 页码:539-545
ISSN号	1006-687X
英文摘要	The goal of this study was to investigate the role of Wnt signaling in mediating the developmental toxicity induced by lead (Pb) exposure using zebrafish (Danio rerio) embryos. Zebrafish embryos were randomly divided into 10 groups. Pb (0, 6, 12, 24, 48 [mu]mol/L) were respectively exposure to zebrafish embryos for 4-144 h post fertilization, with or without HLY78, an activator of the Wnt signaling pathway (5 [mu]mol/L). The expression of wnt3a was detected using quantitative real-time PCR (qRT-PCR). Pb exposure significantly decreased the expression of wnt3a, and supplementation with HLY78 was able to reduce this effect. Mortality, hatchability, and malformation were observed and recorded. Spontaneous movement, heart rate, and locomotive behavior in larval zebrafish were recorded and analyzed using the Vision XT software. Pb exposure significantly decreased hatchability, heart rate, and swimming activities (P < 0.05). Furthermore, mortality and malformation were significantly increased (P < 0.05). Compared to individual Pb exposure, supplementation with HLY78 significantly increased hatchability, heart rate, and swimming activities (P < 0.05), and the mortality and malformation of larvae were significantly decreased (P < 0.05). However, there was no significant difference in the spontaneous movement of embryos between groups. Overall, Pb exposure induced a significant increase in developmental toxicity and decreased the expression of wnt3a. Activation of Wnt signaling alleviated Pb-exposure induced developmental toxicity. Results in this study allow us to further understand the molecular mechanisms of Pb-induced developmental toxicity, which potentially involves the inhibition of Wnt signaling.
WOS研究方向	Zoology ; Computer Science ; Cardiovascular System & Cardiology ; Reproductive Biology ; Developmental Biology ; Genetics & Heredity ; Pathology ; Environmental Sciences & Ecology
语种	英语
WOS记录号	WOS:ZOOR15512085656
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/141543]
专题	中国科学院近代物理研究所
作者单位	Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, 730000, China
推荐引用方式 GB/T 7714	Yan Xiaotao,Gui Fang,Zhang Jinhua,et al. Effect of Wnt signaling on the zebrafish embryonic developmental toxicity induced by lead exposure.[J]. Chinese Journal of Applied and Environmental Biology,2019,25(3):539-545.
APA	Yan Xiaotao,Gui Fang,Zhang Jinhua,Si Jing,&Zhang Hong.(2019).Effect of Wnt signaling on the zebrafish embryonic developmental toxicity induced by lead exposure..Chinese Journal of Applied and Environmental Biology,25(3),539-545.
MLA	Yan Xiaotao,et al."Effect of Wnt signaling on the zebrafish embryonic developmental toxicity induced by lead exposure.".Chinese Journal of Applied and Environmental Biology 25.3(2019):539-545.