Therapeutic Efficacy of Delta-Like Ligand 4 Gene Vaccine Overexpression on Liver Cancer in Mice

doi:10.1177/1533033820942205

	Therapeutic Efficacy of Delta-Like Ligand 4 Gene Vaccine Overexpression on Liver Cancer in Mice
	Yu, Yi 3; Zhao, Yang 2; Zhou, Guangming 1; Wang, Xiang 3
刊名	TECHNOLOGY IN CANCER RESEARCH & TREATMENT
	2020-11-12
卷号	19 页码:9
关键词	delta-like ligand 4 DNA vaccine anti-tumor effect immunotherapy hepatocellular carcinoma immune response
ISSN号	1533-0346
DOI	10.1177/1533033820942205
通讯作者	Wang, Xiang(wangxiang@lzu.edu.cn)
英文摘要	Delta-like ligand 4 is a notch ligand that is predominantly expressed in the endothelial tip cells and plays essential roles in the regulation of angiogenesis. In this study, we explored the therapeutic effects of delta-like ligand 4 gene vaccine overexpression on the syngeneic model mouse model of liver cancer and the underlying mechanisms. Mouse hepatocellular carcinoma cell line H22-H8D8 was used to generate subcutaneous syngeneic model liver cancer in Kunming mice, and the effects of recombinant plasmid pVAX1 containing delta-like ligand 4 vaccine on tumor growth was examined. Compared to controls, delta-like ligand 4 vaccination reduced syngeneic model tumor size by 70.31% (from 17.11 +/- 9.30 cm(3) to 5.08 +/- 2.75 cm(3), P = .035) and tumor weight by 34.19% (from 6.26 +/- 3.01 g to 4.12 +/- 2.52 g, P = .102), while the mouse survival was significantly increased (from 27.7 +/- 6.0 days to 33.1 +/- 6.1 days, P = .047). High level of delta-like ligand 4 antibody, together with a significantly increased number of CD4(+) and decreased CD8(+) cells were identified in the mouse peripheral blood serum samples after delta-like ligand 4 immunization. In addition, elevated serum levels of interleukin 2, interleukin 4, and interferon gamma were detected in the delta-like ligand 4-vaccinated mice when compared to the controls. Further studies have revealed increased CD31 and decreased Ki67 expression in the syngeneic model tumor tissues of vaccinated mice. Taken together, our studies suggest that delta-like ligand 4 gene vaccine can inhibit the growth of hepatocellular carcinoma in mice through inhibiting tumor angiogenesis and boosting antitumor immune responses. Hence, delta-like ligand 4 gene vaccination may be a promising strategy for the treatment of transplanted liver cancer.
资助项目	National Science Foundation of China[30571860]
WOS研究方向	Oncology
语种	英语
出版者	SAGE PUBLICATIONS INC
WOS记录号	WOS:000593483400001
资助机构	National Science Foundation of China
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/139461]
专题	中国科学院近代物理研究所
通讯作者	Wang, Xiang
作者单位	1.Chinese Acad Sci, Inst Modem Phys, Dept Space Radiobiol, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Peoples R China 2.Lanzhou Univ, Hosp 2, Key Lab Digest Syst Tumors, Lanzhou, Gansu, Peoples R China 3.Lanzhou Univ, Hosp 2, Key Lab Digest Dis, Lanzhou, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Yu, Yi,Zhao, Yang,Zhou, Guangming,et al. Therapeutic Efficacy of Delta-Like Ligand 4 Gene Vaccine Overexpression on Liver Cancer in Mice[J]. TECHNOLOGY IN CANCER RESEARCH & TREATMENT,2020,19:9.
APA	Yu, Yi,Zhao, Yang,Zhou, Guangming,&Wang, Xiang.(2020).Therapeutic Efficacy of Delta-Like Ligand 4 Gene Vaccine Overexpression on Liver Cancer in Mice.TECHNOLOGY IN CANCER RESEARCH & TREATMENT,19,9.
MLA	Yu, Yi,et al."Therapeutic Efficacy of Delta-Like Ligand 4 Gene Vaccine Overexpression on Liver Cancer in Mice".TECHNOLOGY IN CANCER RESEARCH & TREATMENT 19(2020):9.