A population balance model describing the cell cycle dynamics of myeloma cell cultivation

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	A population balance model describing the cell cycle dynamics of myeloma cell cultivation
	Liu, Yuan-Hua ; Bi, Jing-Xiu ; Zeng, An-Ping ; Yuan, Jing-Qi
刊名	BIOTECHNOLOGY PROGRESS
	2007-09-01
卷号	23 期号:5 页码:1198-1209
关键词	mammalian suspension cultures amino-acid-concentrations numerical-solution mathematical-model flow-cytometry volume distributions restriction point growth metabolism hybridoma
ISSN号	8756-7938
其他题名	Biotechnol. Prog.
中文摘要	A multi-staged population balance model is proposed to describe the cell cycle dynamics of myeloma cell cultivation. In this model, the cell cycle is divided into three stages, i.e., G1, S, and G2M phases. Both DNA content and cell volume are used to differentiate each cell from other cells of the population. The probabilities of transition from G1 to S and division of G2M are assumed to be dependent on cell volume, and transition probability from S to G2M is determined by DNA content. The model can be used to simulate the dynamics of DNA content and cell volume distributions, phase fractions, and substrate and byproduct concentrations, as well as cell densities. Measurements from myeloma cell cultivations, especially the FACS data with respect to DNA distribution and cell fractions in different stages, are employed for model validation.
英文摘要	A multi-staged population balance model is proposed to describe the cell cycle dynamics of myeloma cell cultivation. In this model, the cell cycle is divided into three stages, i.e., G1, S, and G2M phases. Both DNA content and cell volume are used to differentiate each cell from other cells of the population. The probabilities of transition from G1 to S and division of G2M are assumed to be dependent on cell volume, and transition probability from S to G2M is determined by DNA content. The model can be used to simulate the dynamics of DNA content and cell volume distributions, phase fractions, and substrate and byproduct concentrations, as well as cell densities. Measurements from myeloma cell cultivations, especially the FACS data with respect to DNA distribution and cell fractions in different stages, are employed for model validation.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Food Science & Technology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Food Science & Technology
关键词[WOS]	MAMMALIAN SUSPENSION CULTURES ; AMINO-ACID-CONCENTRATIONS ; NUMERICAL-SOLUTION ; MATHEMATICAL-MODEL ; FLOW-CYTOMETRY ; VOLUME DISTRIBUTIONS ; RESTRICTION POINT ; GROWTH ; METABOLISM ; HYBRIDOMA
收录类别	SCI
原文出处	://WOS:000250010000030
语种	英语
WOS记录号	WOS:000250010000030
公开日期	2013-10-15
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3399]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Shanghai Jiao Tong Univ, Dept Automat, Shanghai 200240, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 3.Hamburg Univ Technol TUHH, Inst Bioproc & Biosyst Engn, D-21073 Hamburg, Germany 4.Helmholtz Ctr Infektionsforsch, D-38124 Braunschweig, Germany
推荐引用方式 GB/T 7714	Liu, Yuan-Hua,Bi, Jing-Xiu,Zeng, An-Ping,et al. A population balance model describing the cell cycle dynamics of myeloma cell cultivation[J]. BIOTECHNOLOGY PROGRESS,2007,23(5):1198-1209.
APA	Liu, Yuan-Hua,Bi, Jing-Xiu,Zeng, An-Ping,&Yuan, Jing-Qi.(2007).A population balance model describing the cell cycle dynamics of myeloma cell cultivation.BIOTECHNOLOGY PROGRESS,23(5),1198-1209.
MLA	Liu, Yuan-Hua,et al."A population balance model describing the cell cycle dynamics of myeloma cell cultivation".BIOTECHNOLOGY PROGRESS 23.5(2007):1198-1209.