Molecular cloning, characterization and expression analysis of CbPLD delta gene from Chorispora bungeana in low temperature

doi:10.1016/j.cryobiol.2020.11.016

	Molecular cloning, characterization and expression analysis of CbPLD delta gene from Chorispora bungeana in low temperature
	Yang, Ning 1; Liu, Bo 1; Yang, Pengjun 1; Chen, Xia 1; Li, Hui 1; Wang, Wei 1; Li, Qiaoxia 1; Wang, Shuyang 2
刊名	CRYOBIOLOGY
	2021-02-01
卷号	98 页码:119-126
关键词	CbPLD delta Clone Transgene Characterization Expression analysis Low temperature stress Chorispora bungeana
ISSN号	0011-2240
DOI	10.1016/j.cryobiol.2020.11.016
通讯作者	Yang, Ning(xbsd-yn@163.com) ; Wang, Shuyang(wangsy@impcas.ac.cn)
英文摘要	Chorispora bungeana (C. bungeana) is a rare alpine subnival species that is highly tolerant to low temperature stress. Phospholipase D (PLD) is a key enzyme involved in membrane phospholipid catabolism during plant growth and the stress response. In this study, one member of CbPLD gene family, CbPLD delta, was cloned from C. bungeana and was introduced into tobacco. This gene encodes an 864-amino acid protein with two catalytic HxKxxxxD motifs which are essential for phospholipase D activity. After the CbPLD delta gene is fused with the vector containing the GFP tag, subcellular localization showed that CbPLD delta was predominately located in the cell membrane. RT-qPCR and histochemical GUS assays showed that CbPLD delta gene was induced by low temperature and expressed predominantly in leaf and root. Compared with wild-type tobacco, CbPLD delta transgenic tobacco showed higher activities of antioxidant enzymes, and lower levels of malonidiadehyde and electrolyte leakage under low temperature stress. These results reflected that CbPLD delta is involved in the response to low temperature stress, and has the potential to improve the low temperature tolerance of plants.
资助项目	National Natural Science Foundation of China[31660116] ; National Natural Science Foundation of China[31960061] ; foundation of the Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations[lzujbky-2018-kb05]
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Physiology
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000618717800017
资助机构	National Natural Science Foundation of China ; foundation of the Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/137906]
专题	中国科学院近代物理研究所
通讯作者	Yang, Ning; Wang, Shuyang
作者单位	1.Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Yang, Ning,Liu, Bo,Yang, Pengjun,et al. Molecular cloning, characterization and expression analysis of CbPLD delta gene from Chorispora bungeana in low temperature[J]. CRYOBIOLOGY,2021,98:119-126.
APA	Yang, Ning.,Liu, Bo.,Yang, Pengjun.,Chen, Xia.,Li, Hui.,...&Wang, Shuyang.(2021).Molecular cloning, characterization and expression analysis of CbPLD delta gene from Chorispora bungeana in low temperature.CRYOBIOLOGY,98,119-126.
MLA	Yang, Ning,et al."Molecular cloning, characterization and expression analysis of CbPLD delta gene from Chorispora bungeana in low temperature".CRYOBIOLOGY 98(2021):119-126.