Phenomena and mechanism for separation and recovery of penicillin in ionic liquids aqueous solution

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	Phenomena and mechanism for separation and recovery of penicillin in ionic liquids aqueous solution
	Jiang, Yangyang ; Xia, Hansong ; Guo, Chen ; Mahmood, Iram ; Liu, Huizhou
刊名	INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
	2007-09-12
卷号	46 期号:19 页码:6303-6312
关键词	partition-coefficients biphasic systems 2-phase systems polyethylene-glycol micellar systems polymer systems water surfaces solvents forces
ISSN号	0888-5885
其他题名	Ind. Eng. Chem. Res.
中文摘要	In this paper, an integrated process based on hydrophilic and hydrophobic ionic liquids is proposed to extract penicillin G frorn its fermentation broth and recover it into fresh water. With the aid of buffer salt, hydrophilic ionic liquids [C(4)mim]BF(4)(1-butyl-3-i-nethylimidazolium tetrafluoraborate) could form an ionic liquids aqueous two-phase systern (ILATPS) and extract penicillin into the ionic liquids-rich phase of ILATPS, while leaving miscellaneous proteins in the ionic liquids-poor phase. Subsequently, hydrophobic [C(4)mim]PF(6)(1-butyl-3-methylimidazolium hexafluoraphosphate) is introduced into the ionic liquids-rich phase of ILATPS, which transfers the system into a hydrophobic ionic liquids phase in equilibrium with a water-phase system (HOILWS). The majority of hydrophilic [C(4)mim]BF4 is transferred into the ionic liquids-rich phase of HOILWS, leaving most of the penicillin in the conjugated water phase. In comparison with the butyl acetate/water system or the polymer- aqueous two-phase system, the integrated ionic liquids system shows several advantages: (1) Penicillin is efficiently extracted into the ionic liquids-rich phase at neutral pH, so the protein emulsification met in the organic solvent system is avoided. (2) Hydrophobic ionic liquids could separate hydrophilic ionic liquids away from the penicillin-containing aqueous phase. Consequently, the trouble for recovering the phase-forming material in the polymer- aqueous two-phase system is overcome. Moreover, the experimental results suggest that the partitioning behaviors of penicillin in ILATPS and HOILWS are different. In ILATPS, the logarithm of the partitioning ratio of penicillin (In K) increases proportionally with the concentration difference of ionic liquids between ionic liquids-rich and liquids-poor phases (A[ionic liquids]), and the partitioning ratio exceeds 1000 at A[ionic liquids] = 2.5 mol/L. While in HOILWS, In K decreases monotonically with A[ionic liquids], and more than 90% penicillin could be recovered from the ionic liquids-rich phase of ILATPS when the mole ratio of [C(4)mim]PF(6)/[C(4)mim]BF(4) exceeds 1. Moreover, the partitioning ratio approaches that in the [C(4)mim]PF(6)/water systern when the [C(4)mim]PF(6)/[C(4)mim]BF(4) ratio in HOILWS is sufficiently large. A modified Flory-Huggins model is proposed to elucidate the partitioning behaviors in the two ionic liquids systems, which emphasizes the key role of energic equilibrium in determining In K ionic liquids solution. In ILATPS, the distance between ionic liquids aggregates is relatively large, which makes the long-range "selfenergy" dominating the partitioning behaviors of penicillin. In contrast, in HOILWS the distance between ionic liquids aggregates is greatly suppressed, which magnifies the entropic loss and hydration repulsion between ionic liquids aggregates and makes them as determinants for the partitioning of penicillin in HOILWS. The conclusions from this model are validated by a series of experimental observations, including the salt's type and concentration, hydrophobic/hydrophilic ionic liquids ratio, and ionic liquids species.
英文摘要	In this paper, an integrated process based on hydrophilic and hydrophobic ionic liquids is proposed to extract penicillin G frorn its fermentation broth and recover it into fresh water. With the aid of buffer salt, hydrophilic ionic liquids [C(4)mim]BF(4)(1-butyl-3-i-nethylimidazolium tetrafluoraborate) could form an ionic liquids aqueous two-phase systern (ILATPS) and extract penicillin into the ionic liquids-rich phase of ILATPS, while leaving miscellaneous proteins in the ionic liquids-poor phase. Subsequently, hydrophobic [C(4)mim]PF(6)(1-butyl-3-methylimidazolium hexafluoraphosphate) is introduced into the ionic liquids-rich phase of ILATPS, which transfers the system into a hydrophobic ionic liquids phase in equilibrium with a water-phase system (HOILWS). The majority of hydrophilic [C(4)mim]BF4 is transferred into the ionic liquids-rich phase of HOILWS, leaving most of the penicillin in the conjugated water phase. In comparison with the butyl acetate/water system or the polymer- aqueous two-phase system, the integrated ionic liquids system shows several advantages: (1) Penicillin is efficiently extracted into the ionic liquids-rich phase at neutral pH, so the protein emulsification met in the organic solvent system is avoided. (2) Hydrophobic ionic liquids could separate hydrophilic ionic liquids away from the penicillin-containing aqueous phase. Consequently, the trouble for recovering the phase-forming material in the polymer- aqueous two-phase system is overcome. Moreover, the experimental results suggest that the partitioning behaviors of penicillin in ILATPS and HOILWS are different. In ILATPS, the logarithm of the partitioning ratio of penicillin (In K) increases proportionally with the concentration difference of ionic liquids between ionic liquids-rich and liquids-poor phases (A[ionic liquids]), and the partitioning ratio exceeds 1000 at A[ionic liquids] = 2.5 mol/L. While in HOILWS, In K decreases monotonically with A[ionic liquids], and more than 90% penicillin could be recovered from the ionic liquids-rich phase of ILATPS when the mole ratio of [C(4)mim]PF(6)/[C(4)mim]BF(4) exceeds 1. Moreover, the partitioning ratio approaches that in the [C(4)mim]PF(6)/water systern when the [C(4)mim]PF(6)/[C(4)mim]BF(4) ratio in HOILWS is sufficiently large. A modified Flory-Huggins model is proposed to elucidate the partitioning behaviors in the two ionic liquids systems, which emphasizes the key role of energic equilibrium in determining In K ionic liquids solution. In ILATPS, the distance between ionic liquids aggregates is relatively large, which makes the long-range "selfenergy" dominating the partitioning behaviors of penicillin. In contrast, in HOILWS the distance between ionic liquids aggregates is greatly suppressed, which magnifies the entropic loss and hydration repulsion between ionic liquids aggregates and makes them as determinants for the partitioning of penicillin in HOILWS. The conclusions from this model are validated by a series of experimental observations, including the salt's type and concentration, hydrophobic/hydrophilic ionic liquids ratio, and ionic liquids species.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Engineering, Chemical
研究领域[WOS]	Engineering
关键词[WOS]	PARTITION-COEFFICIENTS ; BIPHASIC SYSTEMS ; 2-PHASE SYSTEMS ; POLYETHYLENE-GLYCOL ; MICELLAR SYSTEMS ; POLYMER SYSTEMS ; WATER ; SURFACES ; SOLVENTS ; FORCES
收录类别	SCI
原文出处	://WOS:000249290000032
语种	英语
WOS记录号	WOS:000249290000032
公开日期	2013-10-15
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3351]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Yangyang,Xia, Hansong,Guo, Chen,et al. Phenomena and mechanism for separation and recovery of penicillin in ionic liquids aqueous solution[J]. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,2007,46(19):6303-6312.
APA	Jiang, Yangyang,Xia, Hansong,Guo, Chen,Mahmood, Iram,&Liu, Huizhou.(2007).Phenomena and mechanism for separation and recovery of penicillin in ionic liquids aqueous solution.INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,46(19),6303-6312.
MLA	Jiang, Yangyang,et al."Phenomena and mechanism for separation and recovery of penicillin in ionic liquids aqueous solution".INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH 46.19(2007):6303-6312.