Synergism between corn stover protein and cellulase

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	Synergism between corn stover protein and cellulase
	Han, Yejun; Chen, HongZhang
刊名	ENZYME AND MICROBIAL TECHNOLOGY
	2007-10-01
卷号	41 期号:5 页码:638-645
关键词	synergism hydrogen bonds cellulose cellulase cell wall protein purification
ISSN号	0141-0229
其他题名	Enzyme Microb. Technol.
中文摘要	A cellulase synergistic protein named Zea h was purified from fresh postharvest corn stover through sequential treatments. The purified Zea h was homogeneous by PAGE and SDS-PAGE examination, indicating its purity and molecular weight (M,,) of approximately 55.78 kDa. Comparing with cellulase (0.042 FPU) was applied alone in hydrolysis of filter paper, when Zea h (6 mu g) and cellulase were applied together, the hydrolysis rate was increased by a factor of 2 within 1 h, and the total cellulose conversion was increased by a factor of 3 during the 24 It's hydrolysis. Thermal stability analysis revealed that 50.2% of the synergetic activity was recovered when Zea h was incubated in water for 30 min at 100 degrees C, yet the protein was deactivated in high pressure steam (160 degrees C) for 5 min. Studies on the mechanisms of the synergism between Zea h and cellulase showed that, Zea h could increase the adsorption of cellulase onto substrate, and decrease the hydrogen-bond intensity and CrI of substrate. Yet Zea It has no cellulase activity, and has little effect on cellulase stability under the conditions of hydrolysis. It could be concluded that Zea It may modify the structure of filter paper through weakening or rupturing hydrogen bonds, and more it more accessible to cellulase. The unique property of Zea It provides an opportunity for decreasing enzyme loading while retaining the same degree of hydrolysis. (C) 2007 Elsevier Inc. All rights reserved.
英文摘要	A cellulase synergistic protein named Zea h was purified from fresh postharvest corn stover through sequential treatments. The purified Zea h was homogeneous by PAGE and SDS-PAGE examination, indicating its purity and molecular weight (M,,) of approximately 55.78 kDa. Comparing with cellulase (0.042 FPU) was applied alone in hydrolysis of filter paper, when Zea h (6 mu g) and cellulase were applied together, the hydrolysis rate was increased by a factor of 2 within 1 h, and the total cellulose conversion was increased by a factor of 3 during the 24 It's hydrolysis. Thermal stability analysis revealed that 50.2% of the synergetic activity was recovered when Zea h was incubated in water for 30 min at 100 degrees C, yet the protein was deactivated in high pressure steam (160 degrees C) for 5 min.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	VISCOSE-TYPE FIBERS ; ENZYMATIC-HYDROLYSIS ; SODIUM-HYDROXIDE ; HYDROGEN-BONDS ; CARBON-DIOXIDE ; FTIR ANALYSIS ; IR-SPECTRA ; EXPANSINS ; SPECTROSCOPY ; DISRUPTION
收录类别	SCI
原文出处	://WOS:000249224400013
语种	英语
WOS记录号	WOS:000249224400013
公开日期	2013-10-15
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3324]
专题	过程工程研究所_研究所（批量导入）
作者单位	Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Han, Yejun,Chen, HongZhang. Synergism between corn stover protein and cellulase[J]. ENZYME AND MICROBIAL TECHNOLOGY,2007,41(5):638-645.
APA	Han, Yejun,&Chen, HongZhang.(2007).Synergism between corn stover protein and cellulase.ENZYME AND MICROBIAL TECHNOLOGY,41(5),638-645.
MLA	Han, Yejun,et al."Synergism between corn stover protein and cellulase".ENZYME AND MICROBIAL TECHNOLOGY 41.5(2007):638-645.