Functional Diversification of Kaurene Synthase-Like Genes in Isodon rubescens

doi:10.1038/s41598-017-08511-y

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	Functional Diversification of Kaurene Synthase-Like Genes in Isodon rubescens
	Jin, Baolong ; Cui, Guanghong ; Guo, Juan ; Tang, Jinfu ; Duan, Lixin 2; Lin, Huixin ; Shen, Ye ; Chen, Tong ; Zhang, Huabei ; Huang, Luqi
刊名	PLANT PHYSIOLOGY
	2017
卷号	174 期号:2 页码:943-955
ISSN号	0032-0889
DOI	10.1038/s41598-017-08511-y
文献子类	Article
英文摘要	Ent-kaurene diterpenoids are the largest group of known Isodon diterpenoids. Among them, oridonin is accumulated in the leaves, and is the most frequently studied compound because of its antitumor and antibacterial activities. We have identified five copalyl diphosphate synthase (CPS) and six kaurene synthase-like (KSL) genes by transcriptome profiling of Isodon rubescens leaves. An in vitro assay assigns ten of them to five different diterpene biosynthesis pathways, except IrCPS3 that has a mutation in the catalytic motif. The Lamiaceae-specific clade genes (IrCPS1 and IrCPS2) synthesize the intermediate copalyl diphosphate (normal-CPP), while IrCPS4 and IrCPS5 synthesize the intermediate ent-copalyl diphosphate (ent-CPP). IrKSL2, IrKSL4, and IrKSL5 react with ent-CPP to produce an ent-isopimaradiene-like compound, ent-atiserene and ent-kaurene, respectively. Correspondingly, the Lamiaceae-specific clade genes IrKSL1 or IrKSL3 combined with normal-CPP led to the formation of miltiradiene. The compound then underwent aromatization and oxidization with a cytochrome P450 forming two related compounds, abietatriene and ferruginol, which were detected in the root bark. IrKSL6 reacts with normal-CPP to produce isopimaradiene. IrKSL3 and IrKSL6 have the gamma beta alpha tridomain structure, as these proteins tend to possess the bidomain structure of IrKSL1, highlighting the evolutionary history of KSL gene domain loss and further elucidating chemical diversity evolution from a macroevolutionary stance in Lamiaceae.
学科主题	Science & Technology - Other Topics
电子版国际标准刊号	1532-2548
出版地	ROCKVILLE
WOS关键词	COPALYL DIPHOSPHATE SYNTHASE ; DITERPENE SYNTHASES ; BIOSYNTHESIS ; EVOLUTION ; ACID ; DISCOVERY ; BIOGEOGRAPHY ; MILTIRADIENE ; DIVERGENCE ; RESOLUTION
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000407443300014
资助机构	Chinese National Science Fund for Distinguished Young ScholarsNational Natural Science Foundation of China (NSFC)National Science Fund for Distinguished Young Scholars [81325023] ; Key project at central government level: The ability establishment of sustainable use for valuable Chinese medicine resources [2060302]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22258]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Dao Di Herbs, Beijing 100700, Peoples R China
推荐引用方式 GB/T 7714	Jin, Baolong,Cui, Guanghong,Guo, Juan,et al. Functional Diversification of Kaurene Synthase-Like Genes in Isodon rubescens[J]. PLANT PHYSIOLOGY,2017,174(2):943-955.
APA	Jin, Baolong.,Cui, Guanghong.,Guo, Juan.,Tang, Jinfu.,Duan, Lixin.,...&Huang, Luqi.(2017).Functional Diversification of Kaurene Synthase-Like Genes in Isodon rubescens.PLANT PHYSIOLOGY,174(2),943-955.
MLA	Jin, Baolong,et al."Functional Diversification of Kaurene Synthase-Like Genes in Isodon rubescens".PLANT PHYSIOLOGY 174.2(2017):943-955.