OsMAPK3 Phosphorylates OsbHLH002/OsICE1 and Inhibits Its Ubiquitination to Activate OsTPP1 and Enhances Rice Chilling Tolerance

doi:10.1016/j.soilbio.2017.02.015

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	OsMAPK3 Phosphorylates OsbHLH002/OsICE1 and Inhibits Its Ubiquitination to Activate OsTPP1 and Enhances Rice Chilling Tolerance
	Zhang, Zeyong ; Li, Junhua ; Li, Fei ; Liu, Huanhuan ; Yang, Wensi 2; Chong, Kang 2,3; Xu, Yunyuan
刊名	DEVELOPMENTAL CELL
	2017
卷号	43 期号:6 页码:731+
ISSN号	1534-5807
DOI	10.1016/j.soilbio.2017.02.015
文献子类	Article
英文摘要	Improvement of chilling tolerance is a major target in rice breeding. The signaling pathways regulating chilling consist of complex networks, including key transcription factors and their targets. However, it remains largely unknown how transcription factors are activated by chilling stress. Here, we report that the transcription factor OsbHLH002/OsICE1 is phosphorylated by OsMAPK3 under chilling stress. The osbhlh002-1 knockout mutant and antisense transgenic plants showed chilling hypersensitivity, whereas OsbHLH002-overexpressing plants exhibited enhanced chilling tolerance. OsbHLH002 can directly target OsTPP1, which encodes a key enzyme for trehalose biosynthesis. OsMAPK3 interacts with OsbHLH002 to prevent its ubiquitination by the E3 ligase OsHOS1. Under chilling stress, active OsMAPK3 phosphorylates OsbHLH002, leading to accumulation of phospho-OsbHLH002, which promotes OsTPP1 expression and increases trehalose content and resistance to chilling damage. Taken together, these results indicate that OsbHLH002 is phosphorylated by OsMAPK3, which enhances OsbHLH002 activation to its target OsTPP1 during chilling stress.
学科主题	Agriculture
电子版国际标准刊号	1878-1551
出版地	CAMBRIDGE
WOS关键词	RESPONSIVE GENE-EXPRESSION ; COLD SIGNAL-TRANSDUCTION ; ABIOTIC STRESS TOLERANCE ; ORYZA-SATIVA L. ; TRANSCRIPTION FACTOR ; FREEZING TOLERANCE ; PROTEIN-KINASE ; TREHALOSE-6-PHOSPHATE PHOSPHATASE ; TRANSGENIC RICE ; LOW-TEMPERATURE
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000401218800003
资助机构	Chinese Ministry of AgricultureMinistry of Agriculture, China [2016ZX08009003-002] ; Chinese Academy of SciencesChinese Academy of Sciences [XDA08010205] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31400238]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22227]
专题	中科院植物分子生理学重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Zeyong,Li, Junhua,Li, Fei,et al. OsMAPK3 Phosphorylates OsbHLH002/OsICE1 and Inhibits Its Ubiquitination to Activate OsTPP1 and Enhances Rice Chilling Tolerance[J]. DEVELOPMENTAL CELL,2017,43(6):731+.
APA	Zhang, Zeyong.,Li, Junhua.,Li, Fei.,Liu, Huanhuan.,Yang, Wensi.,...&Xu, Yunyuan.(2017).OsMAPK3 Phosphorylates OsbHLH002/OsICE1 and Inhibits Its Ubiquitination to Activate OsTPP1 and Enhances Rice Chilling Tolerance.DEVELOPMENTAL CELL,43(6),731+.
MLA	Zhang, Zeyong,et al."OsMAPK3 Phosphorylates OsbHLH002/OsICE1 and Inhibits Its Ubiquitination to Activate OsTPP1 and Enhances Rice Chilling Tolerance".DEVELOPMENTAL CELL 43.6(2017):731+.