SUMO E3 Ligases GmSIZ1a and GmSIZ1b regulate vegetative growth in soybean

doi:10.16476/j.pibb.2017.0012

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院植物分子生理学重点实验室

	SUMO E3 Ligases GmSIZ1a and GmSIZ1b regulate vegetative growth in soybean
	Cai, Bin ; Kong, Xiangxiong ; Zhong, Chao 4; Sun, Suli 4; Zhou, Xiao Feng 1; Jin, Yin Hua ; Wang, Youning 3; Li, Xia 3; Zhu, Zhendong 4; Jin, Jing Bo
刊名	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
	2017
卷号	59 期号:1 页码:2-14
ISSN号	1672-9072
DOI	10.16476/j.pibb.2017.0012
文献子类	Article
英文摘要	SIZ1 is a small ubiquitin-related modifier (SUMO) E3 ligase that mediates post-translational SUMO modification of target proteins and thereby regulates developmental processes and hormonal and environmental stress responses in Arabidopsis. However, the role of SUMO E3 ligases in crop plants is largely unknown. Here, we identified and characterized two Glycine max (soybean) SUMO E3 ligases, GmSIZ1a and GmSIZ1b. Expression of GmSIZ1a and GmSIZ1b was induced in response to salicylic acid (SA), heat, and dehydration treatment, but not in response to cold, abscisic acid (ABA), and NaCl treatment. Although GmSIZ1a was expressed at higher levels than GmSIZ1b, both genes encoded proteins with SUMO E3 ligase activity in vivo. Heterologous expression of GmSIZ1a or GmSIZ1b rescued the mutant phenotype of Arabidopsis siz1-2, including dwarfism, constitutively activated expression of pathogen-related genes, and ABA-sensitive seed germination. Simultaneous downregulation of GmSIZ1a and GmSIZ1b (GmSIZ1a/b) using RNA interference (RNAi)-mediated gene silencing decreased heat shock-induced SUMO conjugation in soybean. Moreover, GmSIZ1RNAi plants exhibited reduced plant height and leaf size. However, unlike Arabidopsis siz1-2 mutant plants, flowering time and SA levels were not significantly altered in GmSIZ1RNAi plants. Taken together, our results indicate that GmSIZ1a and GmSIZ1b mediate SUMO modification and positively regulate vegetative growth in soybean.
学科主题	Biochemistry & Molecular Biology ; Biophysics
电子版国际标准刊号	1744-7909
出版地	HOBOKEN
WOS关键词	SALT STRESS RESPONSES ; TRANSCRIPTION FACTOR ; FUNCTIONAL-CHARACTERIZATION ; ARABIDOPSIS ; SIZ1 ; UBIQUITIN ; MODIFIER ; SUMOYLATION ; DROUGHT ; TOLERANCE
语种	英语
出版者	WILEY
WOS记录号	WOS:000397482200005
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471363] ; Ministry of Science and Technology of the People's Republic of ChinaMinistry of Science and Technology, China [2012CB114302] ; National Transgenic Major Program [2009ZX08009-087B, 2009ZX08009-132B] ; Chinese Academy of SciencesChinese Academy of Sciences [XDA08010105]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22077]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Huazhong Agr Univ, Coll Plant Sci & Technol, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China 3.China Agr Univ, Dept Ornamental Hort, Beijing 100193, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Cai, Bin,Kong, Xiangxiong,Zhong, Chao,et al. SUMO E3 Ligases GmSIZ1a and GmSIZ1b regulate vegetative growth in soybean[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2017,59(1):2-14.
APA	Cai, Bin.,Kong, Xiangxiong.,Zhong, Chao.,Sun, Suli.,Zhou, Xiao Feng.,...&Jin, Jing Bo.(2017).SUMO E3 Ligases GmSIZ1a and GmSIZ1b regulate vegetative growth in soybean.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,59(1),2-14.
MLA	Cai, Bin,et al."SUMO E3 Ligases GmSIZ1a and GmSIZ1b regulate vegetative growth in soybean".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 59.1(2017):2-14.