Proteome analysis reveals an energy-dependent central process for Populus x canadensis seed germination

doi:10.1038/srep46106

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	Proteome analysis reveals an energy-dependent central process for Populus x canadensis seed germination
	Zhang, Hong ; Zhou, Ke-Xin ; Wang, Wei-Qing ; Liu, Shu-Jun ; Song, Song-Quan
刊名	JOURNAL OF PLANT PHYSIOLOGY
	2017
卷号	213 页码:134-147
关键词	ABA Cycloheximide Poplar seed Proteome Germination
ISSN号	0176-1617
DOI	10.1038/srep46106
文献子类	Article
英文摘要	Poplar (Populus x canadensis) seeds rapidly germinated in darkness at 10, 15, and 20 degrees C and reached 50% seed germination after about 22, 4.5, and 3.5 h, respectively. Germination of poplar seeds was markedly inhibited by abscisic acid (ABA) at 50 mu M and cycloheximide (CHX) at 100 mu M, and these inhibitive roles were temperature-dependent. In the present study, mature poplar seeds were used to investigate the differentially changed proteome of seeds germinating in water, ABA, and CHX. A total of 130 protein spots showed a significant change (1.5-fold increase/decrease, P < 0.05) in abundance, and 101 protein spots were successfully identified. Most of the proteins were associated with cell defense and rescue (21%), storage proteins (21%), protein synthesis and destination (20%), metabolism (16%), and energy (14%). The germination of poplar seeds is closely related with the increase in those proteins involved in amino acid and lipid metabolism, the tricarboxylic acid cycle and pentose phosphate pathway, protein synthesis and destination, cell defense and rescue, and degradation of storage proteins. ABA and CHX inhibit the germination of poplar seeds by decreasing the protein abundance associated with protein proteolysis, protein folding, and storage proteins. We conclude that poplar seed germination is an energy-dependent active process, and is accompanied by increasing amino acid activation, protein synthesis and destination, as well as cell defense and rescue, and degradation of storage proteins. (C) 2017 Elsevier GmbH. All rights reserved.
学科主题	Science & Technology - Other Topics
电子版国际标准刊号	1618-1328
出版地	MUNICH
WOS关键词	RICE EMBRYO ; GENE-EXPRESSION ; MESSENGER-RNAS ; ARABIDOPSIS ; PROTEINS ; ACID ; DORMANCY ; MOBILIZATION ; ENDOSPERM ; TRANSLATION
语种	英语
出版者	ELSEVIER GMBH
WOS记录号	WOS:000398593300001
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31300529, 31171624] ; Public Welfare Project for Environmental Protection [201409055]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22013]
专题	中科院北方资源植物重点实验室
作者单位	1.[Zhang, Hong 2.Minist Environm Protect, Nanjing Inst Environm Sci, Nanjing 210042, Jiangsu, Peoples R China 3.Liu, Shu-Jun 4.Wang, Wei-Qing 5.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Hong,Zhou, Ke-Xin,Wang, Wei-Qing,et al. Proteome analysis reveals an energy-dependent central process for Populus x canadensis seed germination[J]. JOURNAL OF PLANT PHYSIOLOGY,2017,213:134-147.
APA	Zhang, Hong,Zhou, Ke-Xin,Wang, Wei-Qing,Liu, Shu-Jun,&Song, Song-Quan.(2017).Proteome analysis reveals an energy-dependent central process for Populus x canadensis seed germination.JOURNAL OF PLANT PHYSIOLOGY,213,134-147.
MLA	Zhang, Hong,et al."Proteome analysis reveals an energy-dependent central process for Populus x canadensis seed germination".JOURNAL OF PLANT PHYSIOLOGY 213(2017):134-147.