MYB89 Transcription Factor Represses Seed Oil Accumulation

doi:10.1111/gcb.13783

	MYB89 Transcription Factor Represses Seed Oil Accumulation
	Li, Dong ; Jin, Changyu ; Duan, Shaowei ; Zhu, Yana 1; Qi, Shuanghui ; Liu, Kaige ; Gao, Chenhao ; Ma, Haoli ; Zhang, Meng ; Liao, Yuncheng
刊名	PLANT PHYSIOLOGY
	2017
卷号	173 期号:2 页码:1211-1225
ISSN号	0032-0889
DOI	10.1111/gcb.13783
文献子类	Article
英文摘要	In many higher plants, seed oil accumulation is precisely controlled by intricate multilevel regulatory networks, among which transcriptional regulation mainly influences oil biosynthesis. In Arabidopsis (Arabidopsis thaliana), the master positive transcription factors, WRINKLED1 (WRI1) and LEAFY COTYLEDON1-LIKE (L1L), are important for seed oil accumulation. We found that an R2R3-MYB transcription factor, MYB89, was expressed predominantly in developing seeds during maturation. Oil and major fatty acid biosynthesis in seeds was significantly promoted by myb89-1 mutation and MYB89 knockdown; thus, MYB89 was an important repressor during seed oil accumulation. RNA sequencing revealed remarkable up-regulation of numerous genes involved in seed oil accumulation in myb89 seeds at 12 d after pollination. Posttranslational activation of a MYB89-glucocorticoid receptor fusion protein and chromatin immunoprecipitation assays demonstrated that MYB89 inhibited seed oil accumulation by directly repressing WRI1 and five key genes and by indirectly suppressing L1L and 11 key genes involved in oil biosynthesis during seed maturation. These results help us to understand the novel function of MYB89 and provide new insights into the regulatory network of transcriptional factors controlling seed oil accumulation in Arabidopsis.
学科主题	Biodiversity & Conservation ; Environmental Sciences & Ecology
电子版国际标准刊号	1532-2548
出版地	CARY
WOS关键词	FATTY-ACID BIOSYNTHESIS ; ARABIDOPSIS LEAFY COTYLEDON1 ; TRIACYLGLYCEROL ACCUMULATION ; ACETYL-COENZYME ; GENE-EXPRESSION ; DOMAIN PROTEIN ; BRASSICA-NAPUS ; WRINKLED1 ; MATURATION ; ENCODES
语种	英语
出版者	OXFORD UNIV PRESS INC
WOS记录号	WOS:000410642100029
资助机构	Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31501336] ; Science Fund for the Cultivation of Excellent Youth Scholars [Z109021517] ; Startup Fund for Talents of Northwest AF University [Z111021402]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21995]
专题	文献中心
作者单位	1.Northwest A&F Univ, Coll Agron, Yangling 712100, Shaanxi, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Li, Dong,Jin, Changyu,Duan, Shaowei,et al. MYB89 Transcription Factor Represses Seed Oil Accumulation[J]. PLANT PHYSIOLOGY,2017,173(2):1211-1225.
APA	Li, Dong.,Jin, Changyu.,Duan, Shaowei.,Zhu, Yana.,Qi, Shuanghui.,...&Chen, Mingxun.(2017).MYB89 Transcription Factor Represses Seed Oil Accumulation.PLANT PHYSIOLOGY,173(2),1211-1225.
MLA	Li, Dong,et al."MYB89 Transcription Factor Represses Seed Oil Accumulation".PLANT PHYSIOLOGY 173.2(2017):1211-1225.