Excitation dynamics and relaxation in the major antenna of a marine green alga Bryopsis corticulans

doi:10.1016/j.bbabio.2020.148186

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院光生物学重点实验室

	Excitation dynamics and relaxation in the major antenna of a marine green alga Bryopsis corticulans
	Li, Dan-Hong ; Wang, Wenda ; Zhou, Cuicui ; Zhang, Yan ; Wang, Peng ; Shen, Jian-Ren ; Kuang, Tingyun 4; Zhang, Jian-Ping
刊名	BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
	2020
卷号	1861 期号:5-6
关键词	Carotenoids Marine algae Light harvesting Photoprotection Photosynthesis
ISSN号	0005-2728
DOI	10.1016/j.bbabio.2020.148186
文献子类	Article
英文摘要	The light-harvesting complexes II (LHCIIs) of spinach and Bryopsis corticulans as a green alga are similar in structure, but differ in carotenoid (Car) and chlorophyll (Chl) compositions. Carbonyl Cars siphonein (Spn) and siphonaxanthin (Spx) bind to B. corticulans LHCII likely in the sites as a pair of lutein (Lut) molecules bind to spinach LHCII in the central domain. To understand the light-harvesting and photoprotective properties of the algal LHCII, we compared its excitation dynamics and relaxation to those of spinach LHCII been well documented. It was found that B. corticulans LHCII exhibited a substantially longer chlorophyll (Chl) fluorescence lifetime (4.9 ns vs 4.1 ns) and a 60% increase of the fluorescence quantum yield. Photoexcitation populated (3)Car* equally between Spn and Spx in B. corticulans LHCII, whereas predominantly at Lut620 in spinach LHCII. These results prove the functional differences of the LHCIIs with different Car pairs and Chl a/b ratios: B. corticulans LHCII shows the enhanced blue-green light absorption, the alleviated quenching of 1 Chl, and the dual sites of quenching (3)Chl, which may facilitate its light-harvesting and photoprotection functions. Moreover, for both types of LHCIIs, the triplet excitation profiles revealed the involvement of extra (3)Car* formation mechanisms besides the conventional Chl-to-Car triplet transfer, which are discussed in relation to the ultrafast processes of (1)Chl* quenching. Our experimental findings will be helpful in deepening the understanding of the light harvesting and photoprotection functions of B. corticulans living in the intertidal zone with dramatically changing light condition.
学科主题	Biochemistry & Molecular Biology ; Biophysics
电子版国际标准刊号	1879-2650
出版地	AMSTERDAM
WOS关键词	LIGHT-HARVESTING-COMPLEX ; ENERGY-TRANSFER ; PHOTOSYSTEM-II ; PERIDININ-CHLOROPHYLL ; TRIPLET FORMATION ; CATION FORMATION ; STRUCTURAL BASIS ; SINGLET FISSION ; EXCITED-STATES ; HIGHER-PLANTS
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000523619700004
资助机构	Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [21673288, 31600191] ; National Key Research and Development Program of China [2017YFA0503700] ; CAS Key Research program for Frontier Science [QYZDY-SSW-SMC003]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21829]
专题	中科院光生物学重点实验室
作者单位	1.[Li, Dan-Hong 2.Zhang, Yan 3.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 4.Shen, Jian-Ren 5.Wang, Peng 6.Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China 7.[Wang, Wenda 8.Zhou, Cuicui
推荐引用方式 GB/T 7714	Li, Dan-Hong,Wang, Wenda,Zhou, Cuicui,et al. Excitation dynamics and relaxation in the major antenna of a marine green alga Bryopsis corticulans[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2020,1861(5-6).
APA	Li, Dan-Hong.,Wang, Wenda.,Zhou, Cuicui.,Zhang, Yan.,Wang, Peng.,...&Zhang, Jian-Ping.(2020).Excitation dynamics and relaxation in the major antenna of a marine green alga Bryopsis corticulans.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1861(5-6).
MLA	Li, Dan-Hong,et al."Excitation dynamics and relaxation in the major antenna of a marine green alga Bryopsis corticulans".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1861.5-6(2020).