Ribonucleoside Diphosphate Reductase Plays an Important Role in Patulin Degradation by Enterobacter cloacae subsp. dissolvens

doi:10.1021/acs.jafc.0c01613

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	Ribonucleoside Diphosphate Reductase Plays an Important Role in Patulin Degradation by Enterobacter cloacae subsp. dissolvens
	Xing, Mengyang 2; Li, Boqiang ; Chen, Yong ; Tian, Shiping 2
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2020
卷号	68 期号:18 页码:5232-5240
关键词	patulin fruit-derived food mycotoxin contamination biodegradation ribonucleoside diphosphate reductase Enterobacter cloacae
ISSN号	0021-8561
DOI	10.1021/acs.jafc.0c01613
文献子类	Article
英文摘要	Patulin contamination is a worldwide concern due to its significant impact on human health. Several yeast strains have been screened for patulin biodegradation; however, little information is available on bacterial strains and their mechanism of degradation. In the present study, we isolated a bacterial strain TT-09 and identified it as Enterobacter cloacae subsp. dissolvens based on the BioLog system and 16S rDNA phylogenetic analysis. The strain was demonstrated to be able to transform patulin into E-ascladiol. Isobaric tags for relative and absolute quantitation and reverse transcription quantitative polymerase chain reaction analyses provided evidence that ribonucleoside diphosphate reductase (NrdA), an important enzyme involved in DNA biosynthesis, plays a crucial role in patulin degradation. Deletion of nrdA resulted in a total loss in the ability to degrade patulin in TT-09. These results indicate a new function for NrdA in mycotoxin biodegradation. The present study provides evidence for understanding a new mechanism of patulin degradation and information that can be used to develop new approaches for managing patulin contamination.
学科主题	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
电子版国际标准刊号	1520-5118
出版地	WASHINGTON
WOS关键词	ALKYL HYDROPEROXIDE REDUCTASE ; MYCOTOXIN PATULIN ; ESCHERICHIA-COLI ; DETOXIFICATION ; BIOTRANSFORMATION ; IDENTIFICATION ; RHIZOSPHERE ; EXPRESSION ; MECHANISM ; NITROGEN
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000535173900022
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31930086, 31722043] ; National Key R&D Program of China [2016YFD0400902] ; Youth Innovation Promotion Association, CAS [Y201919]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21753]
专题	中科院北方资源植物重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Inst Bot, Chinese Acad Sci, Innovat Acad Seed Design, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Xing, Mengyang,Li, Boqiang,Chen, Yong,et al. Ribonucleoside Diphosphate Reductase Plays an Important Role in Patulin Degradation by Enterobacter cloacae subsp. dissolvens[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2020,68(18):5232-5240.
APA	Xing, Mengyang,Li, Boqiang,Chen, Yong,&Tian, Shiping.(2020).Ribonucleoside Diphosphate Reductase Plays an Important Role in Patulin Degradation by Enterobacter cloacae subsp. dissolvens.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,68(18),5232-5240.
MLA	Xing, Mengyang,et al."Ribonucleoside Diphosphate Reductase Plays an Important Role in Patulin Degradation by Enterobacter cloacae subsp. dissolvens".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 68.18(2020):5232-5240.