A Clathrin-Related Protein, SCD2/RRP1, Participates in Abscisic Acid Signaling inArabidopsis

doi:10.3389/fpls.2020.00892

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	A Clathrin-Related Protein, SCD2/RRP1, Participates in Abscisic Acid Signaling inArabidopsis
	Hou, Bingzhu 1; Shen, Yuanyue
刊名	FRONTIERS IN PLANT SCIENCE
	2020
卷号	11
关键词	Arabidopsis seed germination seedling growth abscisic acid SCD2 RRP1 PYR1 ABI1
ISSN号	1664-462X
DOI	10.3389/fpls.2020.00892
文献子类	Article
英文摘要	Abscisic acid (ABA) plays important roles in many aspects of plant growth and development, and responses to diverse stresses. Although much progress has been made in understanding the molecular mechanisms of ABA homoeostasis and signaling, the mechanism by which plant cells integrate ABA trafficking and signaling to regulate plant developmental processes is poorly understood. In this study, we usedArabidopsis STOMATAL CYTOKINESIS DEFECTIVE 2/RIPENING-REGULATED PROTEIN 1(SCD2/RRP1) mutants and overexpression plants, in combination with transcriptome and protein-interaction assays, to investigate SCD2/RRP1 involvement in the integration of ABA trafficking and signaling in seed germination and seedling growth. Manipulation ofSCD2/RRP1expression affected ABA sensitivity in seed germination and seedling growth, as well as transcription of several ABA transporter genes and ABA content. RNA-sequencing analysis ofArabidopsistransgenic mutants suggested that SCD2/RRP1 was associated with ABA signalingviaa type 2C protein phosphatase (PP2C) protein. The N- and C-terminal regions of SCD2/RRP1 separately interacted with both PYRABACTIN RESISTANCE 1 (PYR1) and ABA INSENSITIVE 1 (ABI1) on the plasma membrane, and SCD2/RRP1 acted genetically upstream of ABI1. Interestingly, ABA inhibited the interaction of SCD2/RRP1 with ABI1, but did not affect the interaction of SCD2/RRP1 with PYR1. These results suggested that inArabidopsisSCD2/RRP1participates in early seed development and growth potentially through clathrin-mediated endocytosis- and clathrin-coated vesicle-mediated ABA trafficking and signaling. These findings provide insight into the mechanism by which cells regulate plant developmental processes through ABA.
学科主题	Plant Sciences
出版地	LAUSANNE
WOS关键词	CHELATASE H-SUBUNIT ; CELL EXPANSION ; ABA ; BIOSYNTHESIS ; TRANSPORTER ; CYTOKINESIS ; HOMEOSTASIS ; ENDOCYTOSIS ; RECEPTOR ; PATHWAY
WOS研究方向	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000546815300001
资助机构	China National Science FoundationNational Natural Science Foundation of China (NSFC) [31672125] ; National Key Research and Development Program [2018YFD1000200] ; Beijing Natural Science FoundationBeijing Natural Science Foundation [6171001] ; Sichuan Lomon Biotechnology Co. Ltd [2018001]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21687]
专题	中科院植物分子生理学重点实验室
作者单位	1.Beijing Univ Agr, Coll Plant Sci & Technol, Beijing Key Lab Agr Applicat & New Tech, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Hou, Bingzhu,Shen, Yuanyue. A Clathrin-Related Protein, SCD2/RRP1, Participates in Abscisic Acid Signaling inArabidopsis[J]. FRONTIERS IN PLANT SCIENCE,2020,11.
APA	Hou, Bingzhu,&Shen, Yuanyue.(2020).A Clathrin-Related Protein, SCD2/RRP1, Participates in Abscisic Acid Signaling inArabidopsis.FRONTIERS IN PLANT SCIENCE,11.
MLA	Hou, Bingzhu,et al."A Clathrin-Related Protein, SCD2/RRP1, Participates in Abscisic Acid Signaling inArabidopsis".FRONTIERS IN PLANT SCIENCE 11(2020).