Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3

doi:10.1093/pcp/pcaa004

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3
	Xu, Meilong 1,4; Tong, Qian 4; Wang, Yi 4; Wang, Zemin 4; Xu, Guangzhao 4; Elias, Gathunga Kirabi 4; Li, Shaohua 4; Liang, Zhenchang 3
刊名	PLANT AND CELL PHYSIOLOGY
	2020
卷号	61 期号:4 页码:775-786
关键词	Cold and osmotic stresses Grapevine LEA genes Overexpression of VamDHN3 gene Phylogenetic analysis Transcriptomic analysis
ISSN号	0032-0781
DOI	10.1093/pcp/pcaa004
文献子类	Article
英文摘要	Late embryogenesis abundant (LEA) proteins comprise a large family that plays important roles in the regulation of abiotic stress, however, no in-depth analysis of LEA genes has been performed in grapevine to date. In this study, we analyzed a total of 52 putative LEA genes in grapevine at the genomic and transcriptomic level, compiled expression profiles of four selected (V. amurensis) VamLEA genes under cold and osmotic stresses, and studied the potential function of the V. amurensis DEHYDRIN3 (VamDHN3) gene in grapevine callus. The 52 LEA proteins were classified into seven phylogenetic groups. RNA-seq and quantitative real-time PCR results demonstrated that a total of 16 and 23 VamLEA genes were upregulated under cold and osmotic stresses, respectively. In addition, overexpression of VamDHN3 enhanced the stability of the cell membrane in grapevine callus, suggesting that VamDHN3 is involved in osmotic regulation. These results provide fundamental knowledge for the further analysis of the biological roles of grapevine LEA genes in adaption to abiotic stress.
学科主题	Plant Sciences ; Cell Biology
电子版国际标准刊号	1471-9053
出版地	OXFORD
WOS关键词	LATE-EMBRYOGENESIS-ABUNDANT ; HABROCHAITES ENHANCES TOLERANCE ; GENOME-WIDE IDENTIFICATION ; DEHYDRIN GENE ; EXPRESSION ANALYSIS ; ESCHERICHIA-COLI ; OXIDATIVE STRESS ; ABIOTIC STRESSES ; PROTEINS ; OVEREXPRESSION
WOS研究方向	Plant Sciences ; Cell Biology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000553476300011
资助机构	Grape breeding project of Ningxia, China [NXNYYZ201502] ; major science and technology program of Ningxia Hui Autonomous region, China [2016BZ06]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21653]
专题	中科院北方资源植物重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China 3.State Key Lab Seedling Bioengn, Yinchuan 750004, Ningxia, Peoples R China 4.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Beijing Key Lab Grape Sci & Enol,CAS Key Lab Plan, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Xu, Meilong,Tong, Qian,Wang, Yi,et al. Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3[J]. PLANT AND CELL PHYSIOLOGY,2020,61(4):775-786.
APA	Xu, Meilong.,Tong, Qian.,Wang, Yi.,Wang, Zemin.,Xu, Guangzhao.,...&Liang, Zhenchang.(2020).Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3.PLANT AND CELL PHYSIOLOGY,61(4),775-786.
MLA	Xu, Meilong,et al."Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3".PLANT AND CELL PHYSIOLOGY 61.4(2020):775-786.