Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging

doi:10.1186/s12863-020-00891-x

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	Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging
	Kong, Weiwei ; Wang, Liming ; Cao, Pei 4; Li, Xingfeng 1; Ji, Jingjing ; Dong, Puhui ; Yan, Xuefang ; Wang, Chunping ; Wang, Honggang 1; Sun, Jiaqiang 2
刊名	BMC GENETICS
	2020
卷号	21 期号:1
关键词	Wheat lmpa1 Mutant Chromosomal location
ISSN号	1471-2156
DOI	10.1186/s12863-020-00891-x
文献子类	Article
英文摘要	Background Lesion-mimic and premature aging (lmpa) mutantlmpa1was identified from the ethyl methane sulfonate (EMS) mutant library in the bread wheat variety Keda 527 (KD527) background. To reveal the genetic basis oflmpa1mutant, phenotypic observations and analyses of chlorophyll content and photosynthesis were carried out inlmpa1, KD527 and their F(1)and F(2)derivatives. Further, bulked segregation analysis (BSA) in combination with a 660 K SNP array were conducted on the F(2)segregation population oflmpa1/Chinese spring (CS) to locate thelmpa1gene. Results Most agronomic traits oflmpa1were similar to those of KD527 before lesion-like spots appeared. Genetic analysis indicated that the F(1)plants from the crossing oflmpa1and KD527 exhibited thelmpaphenotype and the F(2)progenies showed a segregation of normal (wild type, WT) andlmpa, with the ratios oflmpa: WT = 124:36(chi(2) = 1.008 < =3.841), indicating thatlmpais a dominant mutation. The combination of BSA and the SNP array analysis of CS,lmpa1andlmpa1/CS F2WT pool (50 plants) andlmpapool (50 plants) showed that polymorphic SNPs were enriched on chromosome 5A, within a region of 30-40 Mb, indicating that the wheat premature aging geneLmpa1was probably located on the short arm of chromosome 5A. Conclusions EMS-mutagenized mutantlmpa1 deriving from elite wheat line KD527 conferredlmpa.Lmpaphenotype oflmpa1 mutant is controlled by a single dominant allele designated asLmpa1, which affected wheat growth and development and reduced the thousand grain weight (tgw) of single plant in wheat. The geneLmpa1was tentatively located within the region of 30-40 Mb near to the short arm of chromosome 5A.
学科主题	Genetics & Heredity
出版地	LONDON
WOS关键词	CELL-DEATH ; LEAF SENESCENCE ; POWDERY MILDEW ; BSR-SEQ ; RESISTANCE
WOS研究方向	Genetics & Heredity
语种	英语
出版者	BMC
WOS记录号	WOS:000563546300001
资助机构	National Natural Science Foundation of China-Henan Joint fund [U1304318, U1904108] ; Scientific and technological projects of Henan Province of China [202102110022]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21610]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Beijing 10093, Peoples R China 2.Shandong Agr Univ, Agron Coll, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 10081, Peoples R China 4.Henan Univ Sci & Technol, Agron Coll, Luoyang 471023, Henan, Peoples R China
推荐引用方式 GB/T 7714	Kong, Weiwei,Wang, Liming,Cao, Pei,et al. Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging[J]. BMC GENETICS,2020,21(1).
APA	Kong, Weiwei.,Wang, Liming.,Cao, Pei.,Li, Xingfeng.,Ji, Jingjing.,...&Sun, Jiaqiang.(2020).Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging.BMC GENETICS,21(1).
MLA	Kong, Weiwei,et al."Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging".BMC GENETICS 21.1(2020).