Rhizobia promote the growth of rice shoots by targeting cell signaling, division and expansion

doi:10.1007/s11103-018-0756-3

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	Rhizobia promote the growth of rice shoots by targeting cell signaling, division and expansion
	Wu, Qingqing ; Peng, Xianjun ; Yang, Mingfeng 2; Zhang, Wenpeng ; Dazzo, Frank B.; Uphoff, Norman 4; Jing, Yuxiang ; Shen, Shihua
刊名	PLANT MOLECULAR BIOLOGY
	2018
卷号	97 期号:6 页码:507-523
关键词	Endophytic rhizobia Growth-promotion Computer-assisted microscopy Plant-microbe interaction Rice
ISSN号	0167-4412
DOI	10.1007/s11103-018-0756-3
文献子类	Article
英文摘要	The growth-promotion of rice seedling following inoculation with Sinorhizobium meliloti 1021 was a cumulative outcome of elevated expression of genes that function in accelerating cell division and enhancing cell expansion. Various endophytic rhizobacteria promote the growth of cereal crops. To achieve a better understanding of the cellular and molecular bases of beneficial cereal-rhizobia interactions, we performed computer-assisted microscopy and transcriptomic analyses of rice seedling shoots (Oryza sativa) during early stages of endophytic colonization by the plant growth-promoting Sinorhizobium meliloti 1021. Phenotypic analyses revealed that plants inoculated with live rhizobia had increased shoot height and dry weight compared to control plants inoculated with heat-killed cells of the same microbe. At 6 days after inoculation (DAI) with live cells, the fourth-leaf sheaths showed significant cytological differences including their enlargement of parenchyma cells and reduction in shape complexity. Transcriptomic analysis of shoots identified 2,414 differentially-expressed genes (DEGs) at 1, 2, 5 and 8 DAI: 195, 1390, 1025 and 533, respectively. Among these, 46 DEGs encoding cell-cycle functions were up-regulated at least 3 days before the rhizobia ascended from the roots to the shoots, suggesting that rhizobia are engaged in long-distance signaling events during early stages of this plant-microbe interaction. DEGs involved in phytohormone production, photosynthetic efficiency, carbohydrate metabolism, cell division and wall expansion were significantly elevated at 5 and 8 DAI, consistent with the observed phenotypic changes in rice cell morphology and shoot growth-promotion. Correlation analysis identified 104 height-related DEGs and 120 dry-weight-related DEGs that represent known quantitative-trait loci for seedling vigor and increased plant height. These findings provide multiple evidences of plant-microbe interplay that give insight into the growth-promotion processes associated with this rhizobia-rice beneficial association.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
电子版国际标准刊号	1573-5028
出版地	DORDRECHT
WOS关键词	LEGUMINOSARUM BV TRIFOLII ; ORYZA-SATIVA L. ; NATURAL ENDOPHYTIC ASSOCIATION ; ZEA-MAYS L. ; SINORHIZOBIUM-MELILOTI ; ARABIDOPSIS-THALIANA ; SEEDLING-VIGOR ; INOCULATION ; ROOTS ; EXPRESSION
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000442456800003
资助机构	State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2010CB126503]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20503]
专题	中科院北方资源植物重点实验室
作者单位	1.Cornell Univ, SRI Int Network & Resources Ctr SRI Rice, Ithaca, NY 14853 USA 2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 3.Beijing Agr Univ, Coll Biotechnol, Beijing 102206, Peoples R China 4.Dazzo, Frank B.] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA
推荐引用方式 GB/T 7714	Wu, Qingqing,Peng, Xianjun,Yang, Mingfeng,et al. Rhizobia promote the growth of rice shoots by targeting cell signaling, division and expansion[J]. PLANT MOLECULAR BIOLOGY,2018,97(6):507-523.
APA	Wu, Qingqing.,Peng, Xianjun.,Yang, Mingfeng.,Zhang, Wenpeng.,Dazzo, Frank B..,...&Shen, Shihua.(2018).Rhizobia promote the growth of rice shoots by targeting cell signaling, division and expansion.PLANT MOLECULAR BIOLOGY,97(6),507-523.
MLA	Wu, Qingqing,et al."Rhizobia promote the growth of rice shoots by targeting cell signaling, division and expansion".PLANT MOLECULAR BIOLOGY 97.6(2018):507-523.