The WOX11-LBD16 Pathway Promotes Pluripotency Acquisition in Callus Cells During De Novo Shoot Regeneration in Tissue Culture

doi:10.1093/pcp/pcy010

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	The WOX11-LBD16 Pathway Promotes Pluripotency Acquisition in Callus Cells During De Novo Shoot Regeneration in Tissue Culture
	Liu, Jie 5; Hu, Xiaomei 5; Qin, Peng 4; Prasad, Kalika 3; Hu, Yuxin 1; Xu, Lin
刊名	PLANT AND CELL PHYSIOLOGY
	2018
卷号	59 期号:4 页码:739-748
关键词	Arabidopsis thaliana Callus De novo shoot regeneration LBD16 Pluripotency WOX11
ISSN号	0032-0781
DOI	10.1093/pcp/pcy010
文献子类	Article
英文摘要	De novo shoot regeneration in tissue culture undergoes at least two phases. Explants are first cultured on auxin-rich callus-inducing medium (CIM) to produce a group of pluripotent cells termed callus; the callus is then transferred to cytokinin rich shoot-inducing medium (SIM) to promote the formation of shoot progenitor cells, from which adventitious shoots may differentiate. Here, we show that the Arabidopsis thaliana transcription factor gene LATERAL ORGAN BOUNDARIES DOMAIN16 (LBD16) is involved in pluripotency acquisition in callus cells. LBD16, which is activated by WUSCHEL RELATED HOMEOBOX11 (WOX11), is specifically expressed in the newly formed callus on CIM and its expression decreases quickly when callus is moved to SIM. Blocking the WOX11-LBD16 pathway results in the loss of pluripotency in callus cultured on CIM, leading to shooting defects on SIM. Further analysis showed that LBD16 may function in the establishment of the root primordium-like identity in the newly formed callus, indicating that the root primordium-like identity is the cellular nature of pluripotency in callus cells. Additionally, LBD16 promotes cell division during callus initiation. Our study clarified that the WOX11-LBD16 pathway promotes pluripotency acquisition in callus cells.
学科主题	Plant Sciences ; Cell Biology
电子版国际标准刊号	1471-9053
出版地	OXFORD
WOS关键词	LATERAL ROOT-FORMATION ; ARABIDOPSIS-THALIANA ; PLANT-REGENERATION ; FATE TRANSITION ; ACTIVATION ; PRIMORDIA ; WUSCHEL ; ORGANOGENESIS ; GENES ; NICHE
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000430163300009
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31422005/31630007] ; National Basic Research Program of China [973 Program]National Basic Research Program of China [2014CB943500] ; Key Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [QYZDB-SSW-SMC010] ; Strategic Priority Research Program 'Molecular Mechanism of Plant Growth and Development' of the Chinese Academy of Sciences [XDPB0403]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20456]
专题	中科院植物分子生理学重点实验室
作者单位	1.Indian Inst Sci Educ & Res, Sch Biol, Thiruvananthapuram 695016, Kerala, India 2.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Shanghai Jiao Tong Univ, Dept Instrument Sci & Engn, 800 Dongchuan Rd, Shanghai 200240, Peoples R China 4.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci,Natl Key Lab Pla, Shanghai 200032, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jie,Hu, Xiaomei,Qin, Peng,et al. The WOX11-LBD16 Pathway Promotes Pluripotency Acquisition in Callus Cells During De Novo Shoot Regeneration in Tissue Culture[J]. PLANT AND CELL PHYSIOLOGY,2018,59(4):739-748.
APA	Liu, Jie,Hu, Xiaomei,Qin, Peng,Prasad, Kalika,Hu, Yuxin,&Xu, Lin.(2018).The WOX11-LBD16 Pathway Promotes Pluripotency Acquisition in Callus Cells During De Novo Shoot Regeneration in Tissue Culture.PLANT AND CELL PHYSIOLOGY,59(4),739-748.
MLA	Liu, Jie,et al."The WOX11-LBD16 Pathway Promotes Pluripotency Acquisition in Callus Cells During De Novo Shoot Regeneration in Tissue Culture".PLANT AND CELL PHYSIOLOGY 59.4(2018):739-748.