bHLH92 from sheepgrass acts as a negative regulator of anthocyanin/proanthocyandin accumulation and influences seed dormancy

doi:10.1093/jxb/ery335

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	bHLH92 from sheepgrass acts as a negative regulator of anthocyanin/proanthocyandin accumulation and influences seed dormancy
	Zhao, Pincang 2; Li, Xiaoxia ; Jia, Junting ; Yuan, Guangxiao ; Chen, Shuangyan ; Qi, Dongmei ; Cheng, Liqin ; Liu, Gongshe
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2019
卷号	70 期号:1 页码:269-284
关键词	LcbHLH92 Leymus chinensis proanthocyanidins seed color seed dormancy
ISSN号	0022-0957
DOI	10.1093/jxb/ery335
文献子类	Article
英文摘要	Sheepgrass (Leymus chinensis) is an important native forage grass, and it is widely grown in north China. Differential dormancy exists in sheepgrass germplasms with different seed colors. To decipher and find potential genes underlying this phenomenon, we compared the transcript profiles of yellow seeds with weak dormancy and brown seeds with strong dormancy. We identified a transcription factor gene LcbHLH92 which is negatively correlated with those of anthocyanin/proanthocyanidin-specific pathway genes, anthocyanidin synthase (ANS) and anthocyanidin reductase (ANR). LcbHLH92 had two transcripts, LcbHLH92a and LcbHLH92b, and their expression could be induced by abscisic acid, cold, and NaCI. Overexpression of LcbHLH92a or LcbHLH92b in Arabidopsis significantly inhibited the transcript levels of dihydroflavonol reductase (DFR) and ANS genes in leaves and seeds, which resulted in a decrease in anthocyanins and proanthocyanidins, respectively. Importantly, transgenic Arabidopsis seeds with a yellow color showed a higher germination rate than did the wild-type with a brown seed color. Moreover, LcbHLH92a and LcbHLH92b repressed the transcription of Transparent Testa8, ANS, DFR, and ANR, possibly by elevating the transcript levels of jasmonate-ZIM domain proteins through binding to their promoters. Together, our results demonstrate that LcbHLH92a and LcbHLH92b are negative regulators of the anthocyanin/proanthocyanidin pathway and influence seed dormancy.
学科主题	Plant Sciences
电子版国际标准刊号	1460-2431
出版地	OXFORD
WOS关键词	MYB DOMAIN PROTEIN ; COLOR GENE R ; FLAVONOID BIOSYNTHESIS ; ABSCISIC-ACID ; PROANTHOCYANIDIN BIOSYNTHESIS ; ANTHOCYANIN BIOSYNTHESIS ; TEMPERATURE REGULATION ; STRESS TOLERANCE ; GRAIN DORMANCY ; ARABIDOPSIS
WOS研究方向	Plant Sciences
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000459348500022
资助机构	National Basic Research Program of China ('973' program)National Basic Research Program of China [2014CB138704] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31601996, 31170316] ; Project of Science and Technology Service Network Initiative of the Chinese Academy of Sciences (STS) [KFJ-EW-STS-119] ; Conservation of Species Resources of the Ministry of Agriculture of China [2130135] ; Agricultural Technical Experiment and Demonstration of the Ministry of Agriculture of China [13162130106232052]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/19877]
专题	中科院北方资源植物重点实验室
作者单位	1.Hebei Univ Econ & Business, Coll Management Sci & Engn, Shijiazhuang, Hebei, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Pincang,Li, Xiaoxia,Jia, Junting,et al. bHLH92 from sheepgrass acts as a negative regulator of anthocyanin/proanthocyandin accumulation and influences seed dormancy[J]. JOURNAL OF EXPERIMENTAL BOTANY,2019,70(1):269-284.
APA	Zhao, Pincang.,Li, Xiaoxia.,Jia, Junting.,Yuan, Guangxiao.,Chen, Shuangyan.,...&Liu, Gongshe.(2019).bHLH92 from sheepgrass acts as a negative regulator of anthocyanin/proanthocyandin accumulation and influences seed dormancy.JOURNAL OF EXPERIMENTAL BOTANY,70(1),269-284.
MLA	Zhao, Pincang,et al."bHLH92 from sheepgrass acts as a negative regulator of anthocyanin/proanthocyandin accumulation and influences seed dormancy".JOURNAL OF EXPERIMENTAL BOTANY 70.1(2019):269-284.