Evaluation of DNA barcodes in Codonopsis (Campanulaceae) and in some large angiosperm plant genera

doi:10.1371/journal.pone.0175727

CORC > 植物研究所 > 中国科学院植物研究所 > 系统与进化植物学国家重点实验室

	Evaluation of DNA barcodes in Codonopsis (Campanulaceae) and in some large angiosperm plant genera
	Wang, De-Yi ; Wang, Qiang ; Wang, Ying-Li ; Xiang, Xiao-Guo ; Huang, Lu-Qi ; Jin, Xiao-Hua
刊名	PLOS ONE
	2017
卷号	12 期号:2
ISSN号	1932-6203
DOI	10.1371/journal.pone.0175727
文献子类	Article
英文摘要	DNA barcoding is expected to be one of the most promising tools in biological taxonomy. However, there have been no agreements on which core barcode should be used in plants, especially in species-rich genera with wide geographical distributions. To evaluate their discriminatory power in large genera, four of the most widely used DNA barcodes, including three plastid regions (matK, rbcL, trnH-psbA) and nuclear internal transcribed spacer (nrITS), were tested in seven species-rich genera (Ficus, Pedicularis, Rhodiola, Rhododendron, Viburnum, Dendrobium and Lysimachia) and a moderate size genus, Codonopsis. All of the sequences from the aforementioned seven large genera were downloaded from NCBI. The related barcodes for Codonopsis were newly generated in this study. Genetics distances, DNA barcoding gaps and phylogenetic trees of the four single barcodes and their combinations were calculated and compared in the seven genera. As for single barcode, nrITS has the most variable sites, the clearest intra- and inter-specific divergences and the highest discrimination rates in the seven genera. Among the combinations of barcodes, ITS +matK performed better than all the single barcodes in most cases and even the three- and four-loci combinations in the seven genera. Therefore, we recommend ITS+matK as the core barcodes for large plant genera.
学科主题	Science & Technology - Other Topics
出版地	SAN FRANCISCO
WOS关键词	LAND PLANTS ; IDENTIFICATION ; ORCHIDACEAE ; COMPLEMENTS ; EVOLUTION ; TAXONOMY ; HISTORY ; GENOMES ; PROMISE ; SPACER
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000399875200040
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22302]
专题	系统与进化植物学国家重点实验室
作者单位	1.Baotou Med Coll, Baotou City, Inner Mongolia, Peoples R China 2.[Wang, De-Yi 3.Wang, Qiang 4.Xiang, Xiao-Guo 5.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China 6.Univ Chinese Acad Sci, Beijing, Peoples R China 7.Chinese Acad Sci, SEABRI, Southeast Asia Biodivers Res Inst, Mengla, Yunnan, Peoples R China 8.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, De-Yi,Wang, Qiang,Wang, Ying-Li,et al. Evaluation of DNA barcodes in Codonopsis (Campanulaceae) and in some large angiosperm plant genera[J]. PLOS ONE,2017,12(2).
APA	Wang, De-Yi,Wang, Qiang,Wang, Ying-Li,Xiang, Xiao-Guo,Huang, Lu-Qi,&Jin, Xiao-Hua.(2017).Evaluation of DNA barcodes in Codonopsis (Campanulaceae) and in some large angiosperm plant genera.PLOS ONE,12(2).
MLA	Wang, De-Yi,et al."Evaluation of DNA barcodes in Codonopsis (Campanulaceae) and in some large angiosperm plant genera".PLOS ONE 12.2(2017).