DEVELOPMENT OF MICROSATELLITE MARKERS FOR CYPRIPEDIUM TIBETICUM (ORCHIDACEAE) AND THEIR APPLICABILITY TO TWO RELATED SPECIES

doi:10.3390/f8090344

CORC > 植物研究所 > 中国科学院植物研究所 > 系统与进化植物学国家重点实验室

	DEVELOPMENT OF MICROSATELLITE MARKERS FOR CYPRIPEDIUM TIBETICUM (ORCHIDACEAE) AND THEIR APPLICABILITY TO TWO RELATED SPECIES
	Li, Jing ; Luo, Yibo 1; Xu, Lingling
刊名	APPLICATIONS IN PLANT SCIENCES
	2017
卷号	5 期号:12
关键词	Cypripedium tibeticum genetic diversity microsatellites Orchidaceae
ISSN号	2168-0450
DOI	10.3390/f8090344
文献子类	Article
英文摘要	Premise of the study: To investigate genetic diversity and structure, microsatellite markers were developed and characterized for Cypripedium tibeticum, an endangered orchid species, and their utility tested in two relatives: C. flavum and C. bardolphianum. Methods and Results: Candidate microsatellite loci were isolated based on next-generation sequencing. We identified 20 polymorphic loci with di- or trinucleotide repeats in three populations of C. tibeticum. Genotyping results showed the total number of alleles per locus varied from two to 21, and the observed and expected heterozygosity ranged from 0.261 to 0.967 and 0.441 to 0.960, respectively. Cross-species amplification showed most of the markers were applicable to C. flavum and C. bardolphianum. Conclusions: The 20 polymorphic microsatellite markers developed in this study are useful tools for studying the conservation genetics and phylogeography of C. tibeticum and its related species.
学科主题	Forestry
出版地	HOBOKEN
语种	英语
出版者	WILEY
WOS记录号	WOS:000411523700042
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [NSFC 31300366] ; Natural Science Foundation of Shaanxi ProvinceNatural Science Foundation of Shaanxi Province [2015JQ3091] ; Natural Science Foundation of Xi'an Science and Technology Bureau [CXY1531WL28] ; Scientific Research Foundation of the Education Department of Shaanxi [15JK2146]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22281]
专题	系统与进化植物学国家重点实验室
作者单位	1.Xian Univ, Sch Biol & Environm Engn, 1 Keji Six Rd, Xian 710065, Shaanxi, Peoples R China 2.Chinese Acad Sci, State Key Lab Systemat & Evolutionary Bot, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Li, Jing,Luo, Yibo,Xu, Lingling. DEVELOPMENT OF MICROSATELLITE MARKERS FOR CYPRIPEDIUM TIBETICUM (ORCHIDACEAE) AND THEIR APPLICABILITY TO TWO RELATED SPECIES[J]. APPLICATIONS IN PLANT SCIENCES,2017,5(12).
APA	Li, Jing,Luo, Yibo,&Xu, Lingling.(2017).DEVELOPMENT OF MICROSATELLITE MARKERS FOR CYPRIPEDIUM TIBETICUM (ORCHIDACEAE) AND THEIR APPLICABILITY TO TWO RELATED SPECIES.APPLICATIONS IN PLANT SCIENCES,5(12).
MLA	Li, Jing,et al."DEVELOPMENT OF MICROSATELLITE MARKERS FOR CYPRIPEDIUM TIBETICUM (ORCHIDACEAE) AND THEIR APPLICABILITY TO TWO RELATED SPECIES".APPLICATIONS IN PLANT SCIENCES 5.12(2017).