Identification, genealogical structure and population genetics of S-alleles in Malus sieversii, the wild ancestor of domesticated apple

doi:10.3389/fpls.2017.02182

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	Identification, genealogical structure and population genetics of S-alleles in Malus sieversii, the wild ancestor of domesticated apple
	Ma, X.; Cai, Z.; Liu, W.; Ge, S.; Tang, L.
刊名	HEREDITY
	2017
卷号	119 期号:3 页码:185-196
ISSN号	0018-067X
DOI	10.3389/fpls.2017.02182
文献子类	Article
英文摘要	The self-incompatibility (SI) gene that is specifically expressed in pistils encodes the SI-associated ribonuclease (S-RNase), functioning as the female-specificity determinant of a gametophytic SI system. Despite extensive surveys in Malus domestica, the S-alleles have not been fully investigated for Malus sieversii, the primary wild ancestor of the domesticated apple. Here we screened the M. sieversii S-alleles via PCR amplification and sequencing, and identified 14 distinct alleles in this species. By contrast, nearly 40 are present in its close wild relative, Malus sylvestris. We further sequenced 8 nuclear genes to provide a neutral reference, and investigated the evolution of S-alleles via genealogical and population genetic analyses. Both shared ancestral polymorphism and an excess of non-synonymous substitution were detected in the S-RNases of the tribe Maleae in Rosaceae, indicating the action of long-term balancing selection. Approximate Bayesian Computations based on the reference neutral loci revealed a severe bottleneck in four of the six studied M. sieversii populations, suggesting that the low number of S-alleles found in this species is mainly the result of diversity loss due to a drastic population contraction. Such a bottleneck may lead to ambiguous footprints of ongoing balancing selection detected at the S-locus. This study not only elucidates the constituents and number of S-alleles in M. sieversii but also illustrates the potential utility of S-allele number shifts in demographic inference for self-incompatible plant species.
学科主题	Plant Sciences
电子版国际标准刊号	1365-2540
出版地	LONDON
WOS关键词	SELF-INCOMPATIBILITY POLYMORPHISM ; APPROXIMATE BAYESIAN COMPUTATION ; BALANCING SELECTION ; PAPAVER-RHOEAS ; STATISTICAL PROPERTIES ; PHYLOGENETIC ANALYSIS ; SORBUS-AUCUPARIA ; DIVERSITY ; NUMBER ; EVOLUTION
语种	英语
出版者	SPRINGERNATURE
WOS记录号	WOS:000418584800002
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31301747, 41661010] ; Scientific Research Foundation of Hainan University [kyqd1613, kyqd1617]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22063]
专题	系统与进化植物学国家重点实验室
作者单位	1.Tang, L.] Hainan Univ, Inst Trop Agr & Forestry, Coll Trop Forestry, 58 Renming Rd, Haikou 570228, Hainan, Peoples R China 2.[Ma, X. 3.Tang, L.] Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China 4.Ge, S. 5.Liu, W. 6.[Cai, Z.
推荐引用方式 GB/T 7714	Ma, X.,Cai, Z.,Liu, W.,et al. Identification, genealogical structure and population genetics of S-alleles in Malus sieversii, the wild ancestor of domesticated apple[J]. HEREDITY,2017,119(3):185-196.
APA	Ma, X.,Cai, Z.,Liu, W.,Ge, S.,&Tang, L..(2017).Identification, genealogical structure and population genetics of S-alleles in Malus sieversii, the wild ancestor of domesticated apple.HEREDITY,119(3),185-196.
MLA	Ma, X.,et al."Identification, genealogical structure and population genetics of S-alleles in Malus sieversii, the wild ancestor of domesticated apple".HEREDITY 119.3(2017):185-196.