A Novel N-Methyltransferase in Arabidopsis Appears to Feed a Conserved Pathway for Nicotinate Detoxification among Land Plants and Is Associated with Lignin Biosynthesis

doi:10.1002/2017JG003968

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	A Novel N-Methyltransferase in Arabidopsis Appears to Feed a Conserved Pathway for Nicotinate Detoxification among Land Plants and Is Associated with Lignin Biosynthesis
	Li, Wei 1; Zhang, Fengxia 1; Wu, Ranran 1; Jia, Lijia 2,3; Li, Guosheng 1,4; Guo, Yalong 5; Liu, Cuimin 2,3; Wang, Guodong 1
刊名	PLANT PHYSIOLOGY
	2017
卷号	174 期号:3 页码:1492-1504
ISSN号	0032-0889
DOI	10.1002/2017JG003968
文献子类	Article
英文摘要	The Preiss-Handler pathway, which salvages nicotinate (NA) for NAD synthesis, is an indispensable biochemical pathway in land plants. Various NA conjugations (mainly methylation and glycosylation) have been detected and have long been proposed for NA detoxification in plants. Previously, we demonstrated that NA O-glucosylation functions as a mobilizable storage form for NAD biosynthesis in the Brassicaceae. However, little is known about the functions of other NA conjugations in plants. In this study, we first found that N-methylnicotinate is a ubiquitous NA conjugation in land plants. Furthermore, we functionally identified a novel methyltransferase (At3g53140; NANMT), which is mainly responsible for N-methylnicotinate formation, from Arabidopsis (Arabidopsis thaliana). We also established that trigonelline is a detoxification form of endogenous NA in plants. Combined phylogenetic analysis and enzymatic assays revealed that NA N-methylation activity was likely derived from the duplication and subfunctionalization of an ancestral caffeic acid O-methyltransferase (COMT) gene in the course of land plant evolution. COMT enzymes, which function in S-lignin biosynthesis, also have weak NANMT activity. Our data suggest that NA detoxification conferred by NANMT and COMT might have facilitated the retention of the Preiss-Handler pathway in land plants.
学科主题	Environmental Sciences & Ecology ; Geology
电子版国际标准刊号	1532-2548
出版地	ROCKVILLE
WOS关键词	ACID O-METHYLTRANSFERASE ; CONVERGENT EVOLUTION ; DIPHOSPHOPYRIDINE NUCLEOTIDE ; SUBSTRATE-SPECIFICITY ; TRIGONELLINE ; GERMINATION ; COFFEE ; NAD ; EXPRESSION ; COMPONENTS
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000418086800025
资助机构	National Program on Key Basic Research Projects (973 Program)National Basic Research Program of China [2013CB127000] ; National Natural Sciences Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270336] ; State Key Laboratory of Plant Genomics of China [2014b0227-05]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22028]
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Ctr Mol Agrobiol, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 6.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Li, Wei,Zhang, Fengxia,Wu, Ranran,et al. A Novel N-Methyltransferase in Arabidopsis Appears to Feed a Conserved Pathway for Nicotinate Detoxification among Land Plants and Is Associated with Lignin Biosynthesis[J]. PLANT PHYSIOLOGY,2017,174(3):1492-1504.
APA	Li, Wei.,Zhang, Fengxia.,Wu, Ranran.,Jia, Lijia.,Li, Guosheng.,...&Wang, Guodong.(2017).A Novel N-Methyltransferase in Arabidopsis Appears to Feed a Conserved Pathway for Nicotinate Detoxification among Land Plants and Is Associated with Lignin Biosynthesis.PLANT PHYSIOLOGY,174(3),1492-1504.
MLA	Li, Wei,et al."A Novel N-Methyltransferase in Arabidopsis Appears to Feed a Conserved Pathway for Nicotinate Detoxification among Land Plants and Is Associated with Lignin Biosynthesis".PLANT PHYSIOLOGY 174.3(2017):1492-1504.