Energy response patterns to light spectrum at sex differentiation stages of Drynaria roosii gametophytes

doi:10.1016/j.envexpbot.2020.103996

CORC > 植物研究所 > 中国科学院植物研究所 > 系统与进化植物学国家重点实验室

	Energy response patterns to light spectrum at sex differentiation stages of Drynaria roosii gametophytes
	Li, Jing-Yi ; Zhang, Xian-Chun ; Li, Dong ; Sun, Mei-Yu ; Shi, Lei 3
刊名	ENVIRONMENTAL AND EXPERIMENTAL BOTANY
	2020
卷号	172
关键词	Drynaria roosii gametophyte Energy response Light spectrum Sex differentiation WGCNA
ISSN号	0098-8472
DOI	10.1016/j.envexpbot.2020.103996
文献子类	Article
英文摘要	The environmental light changing, such as light spectrum (LS), influences the balance of energy metabolism and sex allocation based on plant species. However, the molecular mechanism underlying systems energy responses (SERs) linked to sex differentiation of Drynaria roosii gametophytes in response to IS remains the enigma. Here, we used Red-LED-Light (RLL), Blue-LED-Light (BLL) and Fluorescent-Light (FL) regimes to treat D. roosii gametophytes to achieve the knowledge of global expression database. Compared with FL, transcriptional changes showed that RLL reduced mRNA levels of photosynthetic genes and improved mRNA levels of respiratory genes. Obviously, RLL decreased PSII/PSI activities with lower Y(II), Fv/Fm, Y(I) and ETR(I) values, and promoted mitochondria activity with higher respiratory rate. Combined with severer ROS stress, we suggested that RLL caused the energy deficit to gametophytes. However, the impacts of BLL were contrary to RLL. Exactly, 63.75 % of males was induced under RLL vs 12.70 % under FL, whilst 64.50 % of females under BLL vs 48.08 % under FL. Moreover, we applied the WGCNA to cluster IS-induced DEGs into eleven modules, and DEGs in five significant sample-specific modules mainly fell in energy metabolism categorization through KEGG pathway analysis. Networks proved the complicated 'cross-talk' between SER- and TF-associated genes, and revealed hub genes in response to LS. Based on previous experimental evidence, we supposed a link between LS-induced energy metabolism responses and sex differentiation in D. roosii gametophytes, showing that RLL contributed to malebiased sex ratios for less energy production than BLL and FL, while BLL contributed to a female-biased trend. Our work firstly shed light on SERs to LS in D. roosii gametophytes, and provided new insights for understanding the sex differentiation in ferns.
学科主题	Plant Sciences ; Environmental Sciences
电子版国际标准刊号	1873-7307
出版地	OXFORD
WOS关键词	INORGANIC CARBON ACQUISITION ; BLUE-LIGHT ; ECTOCARPUS-SILICULOSUS ; GENE-EXPRESSION ; PHOTOSYSTEM-I ; RED-LIGHT ; PHOTOSYNTHESIS ; QUALITY ; ARABIDOPSIS ; ALLOCATION
WOS研究方向	Plant Sciences ; Environmental Sciences & Ecology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000518704100006
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81573519] ; Key Projects of the Chinese Academy of Sciences [KZCC-EW-103-3]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21873]
专题	系统与进化植物学国家重点实验室
作者单位	1.[Li, Jing-Yi 2.Li, Dong 3.Sun, Mei-Yu 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources & Beijing Bot Garden, Beijing 100093, Peoples R China 5.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Li, Jing-Yi,Zhang, Xian-Chun,Li, Dong,et al. Energy response patterns to light spectrum at sex differentiation stages of Drynaria roosii gametophytes[J]. ENVIRONMENTAL AND EXPERIMENTAL BOTANY,2020,172.
APA	Li, Jing-Yi,Zhang, Xian-Chun,Li, Dong,Sun, Mei-Yu,&Shi, Lei.(2020).Energy response patterns to light spectrum at sex differentiation stages of Drynaria roosii gametophytes.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,172.
MLA	Li, Jing-Yi,et al."Energy response patterns to light spectrum at sex differentiation stages of Drynaria roosii gametophytes".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 172(2020).