Identification of the target genes of AqAPETALA3-3 (AqAP3-3) in Aquilegia coerulea (Ranunculaceae) helps understand the molecular bases of the conserved and nonconserved features of petals

doi:10.1111/nph.16601

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	Identification of the target genes of AqAPETALA3-3 (AqAP3-3) in Aquilegia coerulea (Ranunculaceae) helps understand the molecular bases of the conserved and nonconserved features of petals
	Jiang, Yongchao 1; Wang, Meimei 1; Zhang, Rui ; Xie, Jinghe 1; Duan, Xiaoshan ; Shan, Hongyan ; Xu, Guixia ; Kong, Hongzhi 1
刊名	NEW PHYTOLOGIST
	2020
卷号	227 期号:4 页码:1235-1248
关键词	Aquilegia binding sites ChIP-seq floral organ identity genes petals target genes
ISSN号	0028-646X
DOI	10.1111/nph.16601
文献子类	Article
英文摘要	Identification and comparison of the conserved and variable downstream genes of floral organ identity regulators are critical to understanding the mechanisms underlying the commonalities and peculiarities of floral organs. Yet, because of the lack of studies in nonmodel species, a general picture of the regulatory evolution between floral organ identity genes and their targets is still lacking. Here, by conducting extensive chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), electrophoretic mobility shift assay and bioinformatic analyses, we identify and predict the target genes of a petal identity gene, AqAPETALA3-3 (AqAP3-3), in Aquilegia coerulea (Ranunculaceae) and compare them with those of its counterpart in Arabidopsis thaliana, AP3. In total, 7049 direct target genes are identified for AqAP3-3, of which 2394 are highly confident and 1085 are shared with AP3. Gene Ontology enrichment analyses further indicate that conserved targets are largely involved in the formation of identity-related features, whereas nonconserved targets are mostly required for the formation of species-specific features. These results not only help understand the molecular bases of the conserved and nonconserved features of petals, but also pave the way to studying the regulatory evolution between floral organ identity genes and their targets.
学科主题	Plant Sciences
电子版国际标准刊号	1469-8137
出版地	HOBOKEN
WOS关键词	MADS-BOX GENES ; FLORAL ORGAN IDENTITY ; TRANSCRIPTION FACTOR ; CHIP-SEQ ; ARABIDOPSIS PETAL ; HOMEOBOX GENES ; DNA-BINDING ; EVOLUTION ; APETALA3 ; PROTEIN
WOS研究方向	Plant Sciences
语种	英语
出版者	WILEY
WOS记录号	WOS:000533141000001
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31930008] ; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB27010304]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21761]
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Yongchao,Wang, Meimei,Zhang, Rui,et al. Identification of the target genes of AqAPETALA3-3 (AqAP3-3) in Aquilegia coerulea (Ranunculaceae) helps understand the molecular bases of the conserved and nonconserved features of petals[J]. NEW PHYTOLOGIST,2020,227(4):1235-1248.
APA	Jiang, Yongchao.,Wang, Meimei.,Zhang, Rui.,Xie, Jinghe.,Duan, Xiaoshan.,...&Kong, Hongzhi.(2020).Identification of the target genes of AqAPETALA3-3 (AqAP3-3) in Aquilegia coerulea (Ranunculaceae) helps understand the molecular bases of the conserved and nonconserved features of petals.NEW PHYTOLOGIST,227(4),1235-1248.
MLA	Jiang, Yongchao,et al."Identification of the target genes of AqAPETALA3-3 (AqAP3-3) in Aquilegia coerulea (Ranunculaceae) helps understand the molecular bases of the conserved and nonconserved features of petals".NEW PHYTOLOGIST 227.4(2020):1235-1248.