Loss of C-genome-specific markers during transgene introgression from Brassica napusto wild Brassica juncea

doi:10.1111/njb.02794

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	Loss of C-genome-specific markers during transgene introgression from Brassica napusto wild Brassica juncea
	Guan, Zheng-Jun ; Shen, Bao-Cheng ; Wei, Wei ; Tang, Zhi-Xi ; Stewart, C. Neal, Jr.
刊名	NORDIC JOURNAL OF BOTANY
	2020
卷号	38 期号:9
关键词	biosafety Brassica juncea Brassica napus transgene introgression
ISSN号	0107-055X
DOI	10.1111/njb.02794
文献子类	Article
英文摘要	Transgene flow from engineeredBrassica napusto wild weed relatives could potentially have an environmental effect. To evaluate the introgression of transgenicB. napusinto wildBrassica juncea, the hybrid F(1)and backcross progenies derived fromB. juncea(genome constitution AABB) and transgenicB. napus(AACC) crosses were investigated. C-genome-specific simple sequence repeat (SSR) markers corresponding to linkage groups N11-N19 in B. napuswere screened and used to estimate the marker frequency in hybrid F(1)and backcross progenies. C-genome-specific markers could be stably detected in hybrid F-1 and backcross BC1 plants, but were only rarely found in the BC2-BC5 generations. For example, a specific SSR marker for linkage group N12 segregated in BC2 generation but were completely lost in BC3-BC5, while a specific SSR marker of linkage group N15 segregated in BC1, BC2 and BC3 generations and was absent in more advanced backcrossed generations (BC4 and BC5). The results indicate that a certain gene regions inBrassica napusplants are transmitted at a relatively lower frequency to wild relatives, and more rapidly disappeared in subsequent backcross generations. We propose that a foreign gene or transgene that is integrated in the C-chromosome ofBrassica napuscould reduce the risk of introgression in nature.
学科主题	Plant Sciences
电子版国际标准刊号	1756-1051
出版地	HOBOKEN
WOS关键词	OILSEED RAPE ; MUSTARD POPULATIONS ; GENE FLOW ; L. CZERN ; HYBRIDS ; RECOMBINATION ; EXPRESSION ; DIVERSITY ; PERSIST ; FITNESS
WOS研究方向	Plant Sciences
语种	英语
出版者	WILEY
WOS记录号	WOS:000576620000001
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31200422, 31370357] ; Key Disciplines Construction Foundation of the 1331 Project of Shanxi Province [098-091704] ; Natural Science Foundation of Shanxi Province in ChinaNatural Science Foundation of Shanxi Province [201701D121089] ; Yuncheng University [YQ-2014022, XK-2019001]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21565]
专题	植被与环境变化国家重点实验室
作者单位	1.[Stewart, C. Neal, Jr.] Univ Tennessee, Dept Plant Sci, Knoxville, TN USA 2.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, CN-100093 Beijing, Peoples R China 3.Wei, Wei 4.[Shen, Bao-Cheng 5.Yuncheng Univ, Dept Life Sci, Yuncheng, Shanxi, Peoples R China
推荐引用方式 GB/T 7714	Guan, Zheng-Jun,Shen, Bao-Cheng,Wei, Wei,et al. Loss of C-genome-specific markers during transgene introgression from Brassica napusto wild Brassica juncea[J]. NORDIC JOURNAL OF BOTANY,2020,38(9).
APA	Guan, Zheng-Jun,Shen, Bao-Cheng,Wei, Wei,Tang, Zhi-Xi,&Stewart, C. Neal, Jr..(2020).Loss of C-genome-specific markers during transgene introgression from Brassica napusto wild Brassica juncea.NORDIC JOURNAL OF BOTANY,38(9).
MLA	Guan, Zheng-Jun,et al."Loss of C-genome-specific markers during transgene introgression from Brassica napusto wild Brassica juncea".NORDIC JOURNAL OF BOTANY 38.9(2020).