Exon junction complex (EJC) core genes play multiple developmental roles in Physalis floridana

doi:10.1007/s11103-018-0795-9

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	Exon junction complex (EJC) core genes play multiple developmental roles in Physalis floridana
	Gong, Pichang ; Li, Jing 1; He, Chaoying 1
刊名	PLANT MOLECULAR BIOLOGY
	2018
卷号	98 期号:6 页码:545-563
关键词	Pre-mRNA splicing Defense-related process Exon junction complex (EJC) Functional evolution Pollen maturation Physalis floridana
ISSN号	0167-4412
DOI	10.1007/s11103-018-0795-9
文献子类	Article
英文摘要	Key message Molecular and functional characterization of four gene families of the Physalis exon junction complex (EJC) core improved our understanding of the evolution and function of EJC core genes in plants. Abstract The exon junction complex (EJC) plays significant roles in posttranscriptional regulation of genes in eukaryotes. However, its developmental roles in plants are poorly known. We characterized four EJC core genes from Physalis floridana that were named PFMAGO, PFY14, PFeIF4AIII and PFBTZ. They shared a similar phylogenetic topology and were expressed in all examined organs. PFMAGO, PFY14 and PFeIF4AIII were localized in both the nucleus and cytoplasm while PFBTZ was mainly localized in the cytoplasm. No protein homodimerization was observed, but they could form heterodimers excluding the PFY14-PFBTZ heterodimerization. Virus-induced gene silencing (VIGS) of PFMAGO or PFY14 aborted pollen development and resulted in low plant survival due to a leaf-blight-like phenotype in the shoot apex. Carpel functionality was also impaired in the PFY14 knockdowns, whereas pollen maturation was uniquely affected in PFBTZ-VIGS plants. Once PFeIF4AIII was strongly downregulated, plant survival was reduced via a decomposing root collar after flowering and Chinese lantern morphology was distorted. The expression of Physalis orthologous genes in the DYT1-TDF1-AMS-bHLH91 regulatory cascade that is associated with pollen maturation was significantly downregulated in PFMAGO-, PFY14- and PFBTZ-VIGS flowers. Intron-retention in the transcripts of P. floridana dysfunctional tapetum1 (PFDYT1) occurred in these mutated flowers. Additionally, the expression level of WRKY genes in defense-related pathways in the shoot apex of PFMAGO- or PFY14-VIGS plants and in the root collar of PFeIF4AIII-VIGS plants was significantly downregulated. Taken together, the Physalis EJC core genes play multiple roles including a conserved role in male fertility and newly discovered roles in Chinese lantern development, carpel functionality and defense-related processes. These data increase our understanding of the evolution and functions of EJC core genes in plants.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
电子版国际标准刊号	1573-5028
出版地	DORDRECHT
WOS关键词	OSKAR MESSENGER-RNA ; MAGO-NASHI ; TRANSCRIPTION FACTOR ; MOLECULAR CHARACTERIZATION ; EXPRESSION ANALYSIS ; ANTHER DEVELOPMENT ; CRYSTAL-STRUCTURE ; CAP-BINDING ; PROTEIN ; INTERACTS
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000452327100005
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370259, 31525003]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20498]
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Gong, Pichang,Li, Jing,He, Chaoying. Exon junction complex (EJC) core genes play multiple developmental roles in Physalis floridana[J]. PLANT MOLECULAR BIOLOGY,2018,98(6):545-563.
APA	Gong, Pichang,Li, Jing,&He, Chaoying.(2018).Exon junction complex (EJC) core genes play multiple developmental roles in Physalis floridana.PLANT MOLECULAR BIOLOGY,98(6),545-563.
MLA	Gong, Pichang,et al."Exon junction complex (EJC) core genes play multiple developmental roles in Physalis floridana".PLANT MOLECULAR BIOLOGY 98.6(2018):545-563.