Distinguish oral-source VOCs and control their potential impact on breath biomarkers

doi:10.1007/s00216-021-03866-8

	Distinguish oral-source VOCs and control their potential impact on breath biomarkers
	Ge, Dianlong 2,3; Zhou, Jijuan 2,3; Chu, Yajing 2,3; Lu, Yan 2,3; Zou, Xue 3; Xia, Lei 3; Liu, Yawei 3; Huang, Chaoqun 3; Shen, Chengyin 1,3; Zhang, Liwei 1
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2022-01-04
关键词	VOCs Breath biomarker Oral cavity Gargling GC-MS Untargeted analysis
ISSN号	1618-2642
DOI	10.1007/s00216-021-03866-8
通讯作者	Lu, Yan(ylu@cmpt.ac.cn) ; Chu, Yannan(ychu@aiofm.ac.cn)
英文摘要	By means of glass bottle sampling followed by solid-phase microextraction gas chromatography-mass spectrometry (SPME-GC-MS) technique, the change characteristics of volatile organic compounds (VOCs) in breaths, between before gargling and after gargling, were investigated, respectively, in 41 healthy subjects and 50 esophageal cancer patients. Using an untargeted strategy, 143 VOC chromatographic peaks were enrolled in the statistical analysis. Based on the orthogonal partial least squares discriminant analysis (OPLS-DA), the VOC variations after gargling for each breath test group were obtained according to the combined criteria of variable importance in projection (VIP > 1.5), Wilcoxon signed-rank test (P < 0.05), and fold change (FC > 2.0). When gargled, the levels of indole, phenol, 1-propanol, and p-cresol in the breath of healthy people decreased; meanwhile, for esophageal cancer patients, the declined VOCs in breath were indole, phenol, dimethyl disulfide, and p-cresol. Particularly, these substances were previously reported as breath biomarkers in some diseases such as esophageal, gastric, thyroid, breast, oral, and lung cancers, as well as certain non-cancer disorders. The present work indicates that expiratory VOCs involve the prominent oral cavity source, and in the breath biomarkers study, the potential impact that originates from oral volatiles should be considered. In view of the present results, it is also proposed that gargle pretreatment could eliminate possible interference from the oral cavity VOCs that might benefit breath biomarker investigation.
资助项目	National Natural Science Foundation of China[21876176] ; National Natural Science Foundation of China[21777163] ; National Natural Science Foundation of China[21477132] ; National Natural Science Foundation of China[81401756] ; National Natural Science Foundation of China[21705152] ; National Natural Science Foundation of China[22076190] ; Youth Innovation Promotion Association, Chinese Academy of Sciences[2019432] ; President Foundation of Hefei Institute of Physical Sciences of Chinese Academy of Sciences[YZJJZX202009] ; Second Affiliated Hospital of Anhui Medical University[LHJJ2020006] ; Center of Medical Physics and Technology of Hefei Institute of Physical Sciences of Chinese Academy of Sciences[LHJJ2020006] ; Key Program of 13th Five Year Plan, CASHIPS[KP-2017-25] ; Anhui Provincial Key RD Program[202104d07020003]
WOS关键词	VOLATILE ORGANIC-COMPOUNDS ; CHROMATOGRAPHY-MASS-SPECTROMETRY ; EXHALED BREATH ; GAS-CHROMATOGRAPHY ; LUNG-CANCER ; ESOPHAGEAL ; GC ; SPME
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000738486800003
资助机构	National Natural Science Foundation of China ; Youth Innovation Promotion Association, Chinese Academy of Sciences ; President Foundation of Hefei Institute of Physical Sciences of Chinese Academy of Sciences ; Second Affiliated Hospital of Anhui Medical University ; Center of Medical Physics and Technology of Hefei Institute of Physical Sciences of Chinese Academy of Sciences ; Key Program of 13th Five Year Plan, CASHIPS ; Anhui Provincial Key RD Program
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/127171]
专题	中国科学院合肥物质科学研究院
通讯作者	Lu, Yan; Chu, Yannan
作者单位	1.Chinese Acad Sci, Hefei Canc Hosp, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Hlth & Med Technol, Anhui Prov Key Lab Med Phys & Technol, Hefei 230031, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Ge, Dianlong,Zhou, Jijuan,Chu, Yajing,et al. Distinguish oral-source VOCs and control their potential impact on breath biomarkers[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2022.
APA	Ge, Dianlong.,Zhou, Jijuan.,Chu, Yajing.,Lu, Yan.,Zou, Xue.,...&Chu, Yannan.(2022).Distinguish oral-source VOCs and control their potential impact on breath biomarkers.ANALYTICAL AND BIOANALYTICAL CHEMISTRY.
MLA	Ge, Dianlong,et al."Distinguish oral-source VOCs and control their potential impact on breath biomarkers".ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2022).