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	Preparation of uniform-sized PELA microspheres with high encapsulation efficiency of antigen by premix membrane emulsification
	Wei, Qiang1,2; Wei, Wei1,2; Tian, Rui1,2; Wang, Lian-yan1; Su, Zhi-Guo1; Ma, Guang-Hui1
刊名	JOURNAL OF COLLOID AND INTERFACE SCIENCE
	2008-07-15
卷号	323期号:2页码:267-273
关键词	premix membrane emulsification PELA microspheres uniform-sized coalescence encapsulation efficiency
ISSN号	0021-9797
其他题名	J. Colloid Interface Sci.
中文摘要	Relatively uniform-sized poly(lactide-co-ethylene glycol) (PELA) microspheres with high encapsulation efficiency were prepared rapidly by a novel method combining emulsion-solvent extraction and premix membrane emulsification. Briefly, preparation of coarse double emulsions was followed by additional premix membrane emulsification, and antigen-loaded microspheres were obtained by further solidification. Under the optimum condition, the particle size was about 1 pm and the coefficient of variation (CV) value was 18.9%. Confocal laser scanning microscope and flow cytometer analysis showed that the inner droplets were small and evenly dispersed and the antigen was loaded uniformly in each microsphere when sonication technique was occupied to prepare primary emulsion. Distribution pattern of PEG segment played important role on the properties of microspheres. Compared with triblock copolymer PLA-PEG-PLA, the diblock copolymer PLA-mPEG yielded a more stable interfacial layer at the interface of oil and water phase, and thus was more Suitable to stabilize primary emulsion and protect coalescence of inner droplets and external water phase, resulting in high encapsulation efficiency (90.4%). On the other hand, solidification rate determined the time for coalescence during microspheres fabrication, and thus affected encapsulation efficiency. Taken together, improving the polymer properties and solidification rate are considered as two effective strategies to yield high encapsulation. (c) 2008 Elsevier Inc. All rights reserved.
英文摘要	Relatively uniform-sized poly(lactide-co-ethylene glycol) (PELA) microspheres with high encapsulation efficiency were prepared rapidly by a novel method combining emulsion-solvent extraction and premix membrane emulsification. Briefly, preparation of coarse double emulsions was followed by additional premix membrane emulsification, and antigen-loaded microspheres were obtained by further solidification. Under the optimum condition, the particle size was about 1 pm and the coefficient of variation (CV) value was 18.9%. Confocal laser scanning microscope and flow cytometer analysis showed that the inner droplets were small and evenly dispersed and the antigen was loaded uniformly in each microsphere when sonication technique was occupied to prepare primary emulsion. Distribution pattern of PEG segment played important role on the properties of microspheres. Compared with triblock copolymer PLA-PEG-PLA, the diblock copolymer PLA-mPEG yielded a more stable interfacial layer at the interface of oil and water phase, and thus was more Suitable to stabilize primary emulsion and protect coalescence of inner droplets and external water phase, resulting in high encapsulation efficiency (90.4%). On the other hand, solidification rate determined the time for coalescence during microspheres fabrication, and thus affected encapsulation efficiency. Taken together, improving the polymer properties and solidification rate are considered as two effective strategies to yield high encapsulation. (c) 2008 Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Physical
研究领域[WOS]	Chemistry
关键词[WOS]	SOLVENT EVAPORATION METHOD ; POLYMERIC MICROSPHERES ; PLA MICROCAPSULES ; DRUG-DELIVERY ; MICROPARTICLES ; PROTEIN ; NANOPARTICLES ; COPOLYMERS ; SURFACTANTS ; MACROPHAGES
收录类别	SCI
原文出处	://WOS:000256743300011
语种	英语
WOS记录号	WOS:000256743300011
公开日期	2013-10-08
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/2881]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wei, Qiang,Wei, Wei,Tian, Rui,et al. Preparation of uniform-sized PELA microspheres with high encapsulation efficiency of antigen by premix membrane emulsification[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE,2008,323(2):267-273.
APA	Wei, Qiang,Wei, Wei,Tian, Rui,Wang, Lian-yan,Su, Zhi-Guo,&Ma, Guang-Hui.(2008).Preparation of uniform-sized PELA microspheres with high encapsulation efficiency of antigen by premix membrane emulsification.JOURNAL OF COLLOID AND INTERFACE SCIENCE,323(2),267-273.
MLA	Wei, Qiang,et al."Preparation of uniform-sized PELA microspheres with high encapsulation efficiency of antigen by premix membrane emulsification".JOURNAL OF COLLOID AND INTERFACE SCIENCE 323.2(2008):267-273.

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