Ultrasensitive Photoelectrochemical Biosensor Based on Novel Z-Scheme Heterojunctions of Zn-Defective CdS/ZnS for MicroRNA Assay

doi:10.1021/acs.analchem.1c04820

	Ultrasensitive Photoelectrochemical Biosensor Based on Novel Z-Scheme Heterojunctions of Zn-Defective CdS/ZnS for MicroRNA Assay
	Niu, Xiankang 1,2; Zhao, Yan 2; Wang, Fang 2; Wu, Jinghua 2; Qu, Fengli 1,2; Tan, Weihong 1
刊名	ANALYTICAL CHEMISTRY
	2021-12-16
ISSN号	0003-2700
DOI	10.1021/acs.analchem.1c04820
通讯作者	Qu, Fengli(fengliquhn@hotmail.com)
英文摘要	The sensitive and accurate detection of microRNA (miRNA) has meaningful values for clinical diagnosis application as an early stage of tumor markers. Herein, a novel photoelectrochemical (PEC) biosensor was developed for the ultrasensitive and highly selective detection of microRNA-122 (miRNA-122) based on a direct Z-scheme heterojunction of Zn vacancy-mediated CdS/ZnS (CSZS-V-zn). Impressively, the prepared Z-scheme heterojunction nanocomposite with defect level properties could make the photogenerated charges stay at the Zn vacancy defect levels and combine photogenerated holes in the valence bands of CdS, thus significantly achieving a better charge carrier separation efficiency and broadening the absorption of visible light and demonstrating 5-8 times enhancement of PEC response compared to single-component materials. Simultaneously, an exonuclease III (Exo-III)-assisted signal amplification strategy and a strand displacement reaction were combined to improve the conversion efficiency of the target and further increase the detection sensitivity. More importantly, the elaborated biosensor showed ultrasensitive and highly specific detection of the target miRNA-122 over a wide linear range from 10 aM to 100 pM with a low detection limit of 3.3 aM and exhibited enormous potential in the fields of bioanalysis and clinical diagnosis.
资助项目	National Natural Science Foundation of China[21775089] ; National Natural Science Foundation of China[22074080] ; Changjiang Scholar Program of the Ministry of Education of China[Q2019258] ; Taishan Scholar Program of Shandong Province[tsqn201909106]
WOS关键词	PHOTOCATALYSTS ; TIO2
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000733811800001
资助机构	National Natural Science Foundation of China ; Changjiang Scholar Program of the Ministry of Education of China ; Taishan Scholar Program of Shandong Province
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/126944]
专题	中国科学院合肥物质科学研究院
通讯作者	Qu, Fengli
作者单位	1.Chinese Acad Sci, Dept Pathol, Inst Basic Med & Canc, Canc Hosp,Univ Chinese Acad Sci, Hangzhou 310022, Zhejiang, Peoples R China 2.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Niu, Xiankang,Zhao, Yan,Wang, Fang,et al. Ultrasensitive Photoelectrochemical Biosensor Based on Novel Z-Scheme Heterojunctions of Zn-Defective CdS/ZnS for MicroRNA Assay[J]. ANALYTICAL CHEMISTRY,2021.
APA	Niu, Xiankang,Zhao, Yan,Wang, Fang,Wu, Jinghua,Qu, Fengli,&Tan, Weihong.(2021).Ultrasensitive Photoelectrochemical Biosensor Based on Novel Z-Scheme Heterojunctions of Zn-Defective CdS/ZnS for MicroRNA Assay.ANALYTICAL CHEMISTRY.
MLA	Niu, Xiankang,et al."Ultrasensitive Photoelectrochemical Biosensor Based on Novel Z-Scheme Heterojunctions of Zn-Defective CdS/ZnS for MicroRNA Assay".ANALYTICAL CHEMISTRY (2021).