Two coexisting pseudo-mirror heteromolecular telomeric G-quadruplexes in opposite loop progressions differentially recognized by a low equivalent of Thioflavin T

doi:10.1093/nar/gkab755

	Two coexisting pseudo-mirror heteromolecular telomeric G-quadruplexes in opposite loop progressions differentially recognized by a low equivalent of Thioflavin T
	Fu, Wenqiang 2,3; Jing, Haitao 2,3; Xu, Xiaojuan 2,3; Xu, Suping 2; Wang, Tao 2; Hu, Wenxuan 2,3; Li, Huihui 2,3; Zhang, Na 1,2,4,5
刊名	NUCLEIC ACIDS RESEARCH
	2021-10-11
卷号	49
ISSN号	0305-1048
DOI	10.1093/nar/gkab755
通讯作者	Zhang, Na(nazhang@hmfl.cas.cn)
英文摘要	The final 3'-terminal residue of the telomeric DNA G-overhang is inherently less precise. Here, we describe how alteration of the last 3'-terminal base affects the mutual recognition between two different G-rich oligomers of human telomeric DNA in the formation of heteromolecular G-quadruplexes (heteroGC/s). Associations between three- and single-repeat fragments of human telomeric DNA, target d(GGGTTAGGGTTAGGG) and probe d(TAGGGT), in Na+ solution yield two coexisting forms of (3 + 1) hybrid hetero-GQs: the kinetically favourable LLP-form (left loop progression) and the thermodynamically controlled RLP-form (right loop progression). However, only the adoption of a single LLP-form has been previously reported between the same probe d(TAGGGT) and a target variant d(GGGTTAGGGTTAGGG1) having one extra 3'-end thymine. Moreover, the flanking base alterations of short G-rich probe variants also significantly affect the loop progressions of hetero-GQs. Although seemingly two pseudo-mirror counter partners, the RLP-form exhibits a preference over the LLP-form to be recognized by a low equivalent of fluorescence dye thioflavin T (ThT). To a greater extent, ThT preferentially binds to RLP hetero-GO than with the corresponding telomeric DNA duplex context or several other representative unimolecular GQs.
资助项目	National Key Research and Development Program of China[2016YFA0400900] ; National Natural Science Foundation of China[U1932157] ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology[2018ZYFX004]
WOS关键词	MOLECULAR-STRUCTURE DETERMINATION ; K+ SOLUTION ; XPLOR-NIH ; SELECTIVE RECOGNITION ; HEXANUCLEOTIDE REPEAT ; DNA ; NMR ; SEQUENCE ; KINETICS ; BINDING
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000715870700040
资助机构	National Key Research and Development Program of China ; National Natural Science Foundation of China ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/126564]
专题	中国科学院合肥物质科学研究院
通讯作者	Zhang, Na
作者单位	1.Key Lab Anhui Prov High Field Magnet Resonance Im, Hefei 230031, Peoples R China 2.Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Peoples R China 3.Univ Sci & Technol China, Hefei 230026, Peoples R China 4.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Peoples R China 5.High Magnet Field Lab Anhui Prov, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Fu, Wenqiang,Jing, Haitao,Xu, Xiaojuan,et al. Two coexisting pseudo-mirror heteromolecular telomeric G-quadruplexes in opposite loop progressions differentially recognized by a low equivalent of Thioflavin T[J]. NUCLEIC ACIDS RESEARCH,2021,49.
APA	Fu, Wenqiang.,Jing, Haitao.,Xu, Xiaojuan.,Xu, Suping.,Wang, Tao.,...&Zhang, Na.(2021).Two coexisting pseudo-mirror heteromolecular telomeric G-quadruplexes in opposite loop progressions differentially recognized by a low equivalent of Thioflavin T.NUCLEIC ACIDS RESEARCH,49.
MLA	Fu, Wenqiang,et al."Two coexisting pseudo-mirror heteromolecular telomeric G-quadruplexes in opposite loop progressions differentially recognized by a low equivalent of Thioflavin T".NUCLEIC ACIDS RESEARCH 49(2021).