Solid state fermentation of a Mycelia Sterilia laccase using steam-exploded wheat straw

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	Solid state fermentation of a Mycelia Sterilia laccase using steam-exploded wheat straw
	Qiu, Weihua; Chen, Hongzhang
刊名	WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
	2008-02-01
卷号	24 期号:2 页码:219-224
关键词	enzyme composition analysis laccase Mycelia Sterilia YY-5 solid state fermentation steam-explosion intensity steam-exploded wheat straw
ISSN号	0959-3993
其他题名	World J. Microbiol. Biotechnol.
中文摘要	Mycelia Sterilia YY-5, an endophytic fungus isolated from Rhus Chinensis Mill, was used in SSF for laccase production using steam-exploded wheat straw (SEWS). The fermentation period of YY-5 in solid state fermentation (SSF) shortened to 4 days compared with 5 days of submerged liquid fermentation (SmF) and the maximum laccase activity was 678.1 IU g(-1) substrate. The steam-explosion intensity (Log(10)R(0)) of SEWS had a significant effect on the growth of YY-5 and laccase activity, since SEWS could provide enough carbon source for YY-5 and inducers for laccase. The optimum SSF conditions using SEWS with Log(10)R(0) = 3.597 as substrate were: inoculating with liquid inocula, keeping the solid-to-liquid ratio (S/L) for 1:4 and cultivating at 26 degrees C. Under the optimum fermentation condition the laccase activity of YY-5 reached 849.5 +/- 42.5 IU g(-1) substrate. The enzyme composition analysis indicated that laccase was the dominant enzyme of YY-5. Assayed with SDS-PAGE and active PAGE electrophoresis, the molecular weight of YY-5 laccase was approximately 45 kDa.
英文摘要	Mycelia Sterilia YY-5, an endophytic fungus isolated from Rhus Chinensis Mill, was used in SSF for laccase production using steam-exploded wheat straw (SEWS). The fermentation period of YY-5 in solid state fermentation (SSF) shortened to 4 days compared with 5 days of submerged liquid fermentation (SmF) and the maximum laccase activity was 678.1 IU g(-1) substrate. The steam-explosion intensity (Log(10)R(0)) of SEWS had a significant effect on the growth of YY-5 and laccase activity, since SEWS could provide enough carbon source for YY-5 and inducers for laccase. The optimum SSF conditions using SEWS with Log(10)R(0) = 3.597 as substrate were: inoculating with liquid inocula, keeping the solid-to-liquid ratio (S/L) for 1:4 and cultivating at 26 degrees C. Under the optimum fermentation condition the laccase activity of YY-5 reached 849.5 +/- 42.5 IU g(-1) substrate. The enzyme composition analysis indicated that laccase was the dominant enzyme of YY-5. Assayed with SDS-PAGE and active PAGE electrophoresis, the molecular weight of YY-5 laccase was approximately 45 kDa.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	VERSICOLOR ; SUBSTRATE ; CULTURES ; FUNGI
收录类别	SCI
原文出处	://WOS:000252476900010
语种	英语
WOS记录号	WOS:000252476900010
公开日期	2013-10-08
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/2832]
专题	过程工程研究所_研究所（批量导入）
作者单位	Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Qiu, Weihua,Chen, Hongzhang. Solid state fermentation of a Mycelia Sterilia laccase using steam-exploded wheat straw[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2008,24(2):219-224.
APA	Qiu, Weihua,&Chen, Hongzhang.(2008).Solid state fermentation of a Mycelia Sterilia laccase using steam-exploded wheat straw.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,24(2),219-224.
MLA	Qiu, Weihua,et al."Solid state fermentation of a Mycelia Sterilia laccase using steam-exploded wheat straw".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 24.2(2008):219-224.