A simple kinetic model for myeloma cell culture with consideration of lysine limitation

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	A simple kinetic model for myeloma cell culture with consideration of lysine limitation
	Liu, Yuan-Hua 1; Bi, Jing-Xiu 2,3; Zeng, An-Ping 4,5; Yuan, Jing-Qi 1
刊名	BIOPROCESS AND BIOSYSTEMS ENGINEERING
	2008-10-01
卷号	31 期号:6 页码:569-577
关键词	myeloma cell line kinetic model amino acids lysine modeling growth kinetics
ISSN号	1615-7591
其他题名	Bioprocess. Biosyst. Eng.
中文摘要	A simple kinetic model is developed to describe the dynamic behavior of myeloma cell growth and cell metabolism. Glucose, glutamine as well as lysine are considered as growth limiting substrates. The cell growth was restricted as soon as the extracellular lysine is exhausted and then intracellular lysine becomes a growth limiting substrate. In addition, a metabolic regulator model together with the Monod model is used to deal with the growth lag phase after inoculation or feeding. By using these models, concentrations of substrates and metabolites, as well as densities of viable and dead cells are quantitatively described. One batch cultivation and two fed-batch cultivations with pulse feeding of nutrients are used to validate the model.
英文摘要	A simple kinetic model is developed to describe the dynamic behavior of myeloma cell growth and cell metabolism. Glucose, glutamine as well as lysine are considered as growth limiting substrates. The cell growth was restricted as soon as the extracellular lysine is exhausted and then intracellular lysine becomes a growth limiting substrate. In addition, a metabolic regulator model together with the Monod model is used to deal with the growth lag phase after inoculation or feeding. By using these models, concentrations of substrates and metabolites, as well as densities of viable and dead cells are quantitatively described. One batch cultivation and two fed-batch cultivations with pulse feeding of nutrients are used to validate the model.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Biotechnology & Applied Microbiology ; Engineering, Chemical
研究领域[WOS]	Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]	AMINO-ACID-CONCENTRATIONS ; MONOCLONAL-ANTIBODY PRODUCTION ; HYBRIDOMA CELLS ; MATHEMATICAL DESCRIPTIONS ; ENDOTHELIAL-CELLS ; BATCH CULTURE ; CHO-CELLS ; METABOLISM ; GROWTH ; SINGLE
收录类别	SCI
原文出处	://WOS:000258833600007
语种	英语
WOS记录号	WOS:000258833600007
公开日期	2013-10-08
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/2816]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Shanghai Jiao Tong Univ, Dept Automat, Shanghai 200240, Peoples R China 2.Univ Adelaide, Sch Chem Engn, Adelaide, SA 5005, Australia 3.Chinese Acad Sci, Inst Proc Engn, Beijing 100080, Peoples R China 4.Tech Univ Hamburg, Hamburg Univ Technol, Inst Bioproc & Biosyst Engn, D-21073 Hamburg, Germany 5.Helmholtz Zentrum Infekt Forsch, Res Grp Syst Biol, D-38124 Braunschweig, Germany
推荐引用方式 GB/T 7714	Liu, Yuan-Hua,Bi, Jing-Xiu,Zeng, An-Ping,et al. A simple kinetic model for myeloma cell culture with consideration of lysine limitation[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2008,31(6):569-577.
APA	Liu, Yuan-Hua,Bi, Jing-Xiu,Zeng, An-Ping,&Yuan, Jing-Qi.(2008).A simple kinetic model for myeloma cell culture with consideration of lysine limitation.BIOPROCESS AND BIOSYSTEMS ENGINEERING,31(6),569-577.
MLA	Liu, Yuan-Hua,et al."A simple kinetic model for myeloma cell culture with consideration of lysine limitation".BIOPROCESS AND BIOSYSTEMS ENGINEERING 31.6(2008):569-577.