Enzymatic hydrolysis of penicillin for 6-APA production in three-liquid-phase system

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	Enzymatic hydrolysis of penicillin for 6-APA production in three-liquid-phase system
	Jiang, Yangyang 1,2; Xia, Hansong 1; Guo, Chen 1; Mahmood, Iram 1,2; Liu, Huizhou 1
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2008-02-01
卷号	144 期号:2 页码:145-159
关键词	three-liquid-phase system covalent attachment PEO-PPO-PEO polymer (L63) ionic liquids (Cyphos IL-104) penicillin G acylase 6-aminopenicillanic acid (6-APA)
ISSN号	0273-2289
其他题名	Appl. Biochem. Biotechnol.
中文摘要	A dodecane/thermosensitive polymer/water three-liquid-phase system was introduced for enzymatic hydrolysis of penicillin G (Pen G) for 6-aminopenicillanic acid (6-APA). The enzyme was covalently attached to the terminal of PEO-PPO-PEO polymer (L63), which would be transferred into a polymer coacervate phase at high temperature above its "cloud point". 6-APA was primarily resided in the aqueous phase due to its zwitterionic nature. More than 70% phenylacetic acid (PAA) was transferred into the organic phase using trioctylmethylammonium hydroxide and trihexyl-(tetradecyl)phosphonium bis 2,4,4-trimethylpentylphosphinate ionic liquids (Cyphos IL-104) mixture at pH 5.5, while most of Pen G resided in water. As a result, high operational pH was permitted in three-liquid-phase system, which leads to higher enzymatic activity (120 IU at 40 degrees C) and stability (enzymatic half-time up to 55 h at 60 degrees C) in comparison with the value in butyl acetate/water two-phase system. On the other hand, two products in three-liquid-phase system might be automatically separated from the enzyme sphere into different phases at the same time, which facilitated the reaction equilibrium towards the product's side with 6-APA productivity of 80% at 42 degrees C, pH 5.5.
英文摘要	A dodecane/thermosensitive polymer/water three-liquid-phase system was introduced for enzymatic hydrolysis of penicillin G (Pen G) for 6-aminopenicillanic acid (6-APA). The enzyme was covalently attached to the terminal of PEO-PPO-PEO polymer (L63), which would be transferred into a polymer coacervate phase at high temperature above its "cloud point". 6-APA was primarily resided in the aqueous phase due to its zwitterionic nature. More than 70% phenylacetic acid (PAA) was transferred into the organic phase using trioctylmethylammonium hydroxide and trihexyl-(tetradecyl)phosphonium bis 2,4,4-trimethylpentylphosphinate ionic liquids (Cyphos IL-104) mixture at pH 5.5, while most of Pen G resided in water. As a result, high operational pH was permitted in three-liquid-phase system, which leads to higher enzymatic activity (120 IU at 40 degrees C) and stability (enzymatic half-time up to 55 h at 60 degrees C) in comparison with the value in butyl acetate/water two-phase system. On the other hand, two products in three-liquid-phase system might be automatically separated from the enzyme sphere into different phases at the same time, which facilitated the reaction equilibrium towards the product's side with 6-APA productivity of 80% at 42 degrees C, pH 5.5.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	AQUEOUS 2-PHASE SYSTEMS ; 6-AMINOPENICILLANIC ACID ; REACTIVE EXTRACTION ; G ACYLASE ; EQUILIBRIUM ; ANTIBIOTICS ; STABILITY ; KINETICS ; IMMOBILIZATION ; BIOCATALYST
收录类别	SCI
原文出处	://WOS:000252562900005
语种	英语
WOS记录号	WOS:000252562900005
公开日期	2013-10-08
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/2777]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Lab Seperat Sci & Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Yangyang,Xia, Hansong,Guo, Chen,et al. Enzymatic hydrolysis of penicillin for 6-APA production in three-liquid-phase system[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2008,144(2):145-159.
APA	Jiang, Yangyang,Xia, Hansong,Guo, Chen,Mahmood, Iram,&Liu, Huizhou.(2008).Enzymatic hydrolysis of penicillin for 6-APA production in three-liquid-phase system.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,144(2),145-159.
MLA	Jiang, Yangyang,et al."Enzymatic hydrolysis of penicillin for 6-APA production in three-liquid-phase system".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 144.2(2008):145-159.