Liquid-liquid/solid three-phase high-speed counter-current chromatography, a new technique for separation of polyphenols from Geranium wilfordii Maxim

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	Liquid-liquid/solid three-phase high-speed counter-current chromatography, a new technique for separation of polyphenols from Geranium wilfordii Maxim
	Liu, Dan 1,2; Ma, Yan 3; Gu, Ming 1; Janson, Jan-Christer 4; Wang, Changhai 5; Xiao, Hongbin 2
刊名	JOURNAL OF SEPARATION SCIENCE
	2012-08-01
卷号	35 期号:16 页码:2146-2151
关键词	Corilagin Geraniin High-speed counter-current chromatography Liquid-liquid solid three-phase system Polyphenols
ISSN号	1615-9306
通讯作者	Gu, M
英文摘要	High-speed counter-current chromatography using a new liquidliquid/solid three-phase system was used for the separation of the polyphenols corilagin and geraniin from a crude extract of Geranium wilfordii Maxim in one step. The optimized three-phase system was composed of n-hexane/ethyl acetate/methanol/acetic acid/water and to which was added 10-mu m average diameter microspheres of cross-linked 12% agarose at the ratio of 0.2:10:2:1:5 and 0.1 g/mL, respectively. The purities of geraniin and corilagin were 82 and 90%, which were determined by HPLC at 280 nm. A 14.5 and 7 mg of geraniin and corilagin were purified from 160 mg crude extract with the yields of 70 and 78%, respectively.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	LINKED 12-PERCENT AGAROSE ; ONE-STEP PURIFICATION ; BOND ADSORPTION CHROMATOGRAPHY ; SALVIANOLIC ACID-B ; 3,4-DIHYDROXYPHENYLLACTIC ACID ; PHYLLANTHUS-URINARIA ; HYDROLYZABLE TANNINS ; GEL MEDIA ; CORILAGIN ; PROTOCATECHUALDEHYDE
收录类别	SCI
语种	英语
WOS记录号	WOS:000307551300020
公开日期	2013-10-15
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3228]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 3.Liaoning Prov Dalian Ocean Fishery Grp Corp, Dalian, Peoples R China 4.Uppsala Univ, Dept Phys & Analyt Chem, Uppsala, Sweden 5.Nanjing Agr Univ, Nanjing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Dan,Ma, Yan,Gu, Ming,et al. Liquid-liquid/solid three-phase high-speed counter-current chromatography, a new technique for separation of polyphenols from Geranium wilfordii Maxim[J]. JOURNAL OF SEPARATION SCIENCE,2012,35(16):2146-2151.
APA	Liu, Dan,Ma, Yan,Gu, Ming,Janson, Jan-Christer,Wang, Changhai,&Xiao, Hongbin.(2012).Liquid-liquid/solid three-phase high-speed counter-current chromatography, a new technique for separation of polyphenols from Geranium wilfordii Maxim.JOURNAL OF SEPARATION SCIENCE,35(16),2146-2151.
MLA	Liu, Dan,et al."Liquid-liquid/solid three-phase high-speed counter-current chromatography, a new technique for separation of polyphenols from Geranium wilfordii Maxim".JOURNAL OF SEPARATION SCIENCE 35.16(2012):2146-2151.