Novel thermal-sensitive hydrogel enhances both humoral and cell-mediated immune responses by intranasal vaccine delivery

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	Novel thermal-sensitive hydrogel enhances both humoral and cell-mediated immune responses by intranasal vaccine delivery
	Wu, Youbin 1,2; Wu, Shipo 3; Hou, Lihua 3; Wei, Wei 1; Zhou, Meng 1; Su, Zhiguo 1; Wu, Jie 1; Chen, Wei 3; Ma, Guanghui 1
刊名	EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
	2012-08-01
卷号	81 期号:3 页码:486-497
关键词	Quarternized chitosan Hydrogel Thermal sensitive Mucosal immunization Vaccine delivery Ebola
ISSN号	0939-6411
通讯作者	Wu, J
英文摘要	A novel thermal sensitive hydrogel was formulated with N-[(2-hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTCC) and alpha, beta-glycerophosphate (alpha, beta-GP). A serial of hydrogels containing different amount of GP and HTCC with diverse quarternize degree (QD, 41%, 59%, 79.5%, and 99%) were prepared and characterized by rheological method. The hydrogel was subsequently evaluated for intranasal vaccine delivery with adenovirus based Zaire Ebola virus glycoprotein antigen (Ad-GPZ). Results showed that moderate quarternized HTCC (60% and 79.5%) hydrogel/antigen formulations induced highest IgG, IgG1, and IgG2a antibody titers in serum, as well as mucosal IgA responses in lung wash, which may attributed to the prolonged antigen residence time due to the thermal-sensitivity of this hydrogel. Furthermore, CD8(+) splenocytes for IFN-gamma positive cell assay and the release profile of Th1/Th2 type cytokines (IFN-gamma, IL-2, IL-10, and IL-4) showed that hydrogel/Ad-GPZ generated an overwhelmingly enhanced Th1 biased cellular immune response. In addition, this hydrogel displayed low toxicity to nasal tissue and epithelial cells even by frequently intranasal dosing of hydrogel. All these results strongly supported this hydrogel as a safe and effective delivery system for nasal immunization. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Pharmacology & Pharmacy
研究领域[WOS]	Pharmacology & Pharmacy
关键词[WOS]	NASAL DRUG-DELIVERY ; IGG IMMUNOLOGICAL RESPONSES ; CHITOSAN NANOPARTICLES ; INFLUENZA VACCINE ; MUCOSAL SIGA ; EBOLA-VIRUS ; ABSORPTION ENHANCERS ; RHESUS-MONKEYS ; INFECTION ; SYSTEM
收录类别	SCI
语种	英语
WOS记录号	WOS:000307040900004
公开日期	2013-10-14
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3220]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 2.Chinese Acad Sci, Graduated Univ, Beijing, Peoples R China 3.Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogens & Biosecur, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wu, Youbin,Wu, Shipo,Hou, Lihua,et al. Novel thermal-sensitive hydrogel enhances both humoral and cell-mediated immune responses by intranasal vaccine delivery[J]. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS,2012,81(3):486-497.
APA	Wu, Youbin.,Wu, Shipo.,Hou, Lihua.,Wei, Wei.,Zhou, Meng.,...&Ma, Guanghui.(2012).Novel thermal-sensitive hydrogel enhances both humoral and cell-mediated immune responses by intranasal vaccine delivery.EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS,81(3),486-497.
MLA	Wu, Youbin,et al."Novel thermal-sensitive hydrogel enhances both humoral and cell-mediated immune responses by intranasal vaccine delivery".EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS 81.3(2012):486-497.