Enhanced the enzymatic hydrolysis efficiency of wheat straw after combined steam explosion and laccase pretreatment

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	Enhanced the enzymatic hydrolysis efficiency of wheat straw after combined steam explosion and laccase pretreatment
	Qiu, Weihua; Chen, Hongzhang
刊名	BIORESOURCE TECHNOLOGY
	2012-08-01
卷号	118 期号:0 页码:8-12
关键词	Laccase Steam explosion Enzymatic hydrolysis Selective degradation of lignin
ISSN号	0960-8524
通讯作者	Chen, HZ
英文摘要	Laccase, capable of selectively degrading lignin while keeping cellulose intact, has been widely applied for the modification and bio-bleaching of pulp. In this study Sclerotium sp. laccase (MSLac) was employed in combination with steam explosion to evaluate the effect of this treatment on cellulose hydrolysis. Combined steam explosion with laccase pretreatment enhanced the cellulose conversion rate of wheat straw no matter in the case of successive (MSLac-Cel) and simultaneous (MSLac+Cel) MSLac and cellulase hydrolysis. The highest cellulose conversion rate of 84.23% was obtained when steam-exploded wheat straw (SEWS) (1.3 MPa, 5 min) was treated by MSLac+Cel at a laccase loading of 0.55 U g(-1) substrate. FT-IR and SEM analyses indicated that MSLac oxidized the phenol and changed electron configuration of the ring, which contributed to loosening the compact wrap of lignin-carbohydrate complex and consequently enhancing the enzymatic hydrolysis efficiency of cellulose. This article provided a promising method for lignocellulose bio-pretreatment. (C) 2012 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]	BIOETHANOL PRODUCTION ; DELIGNIFICATION ; FERMENTATION ; TECHNOLOGIES ; SOFTWOOD ; XYLANASE ; FIBERS ; SYSTEM
收录类别	SCI
语种	英语
WOS记录号	WOS:000307259600002
公开日期	2013-10-14
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3211]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Qiu, Weihua,Chen, Hongzhang. Enhanced the enzymatic hydrolysis efficiency of wheat straw after combined steam explosion and laccase pretreatment[J]. BIORESOURCE TECHNOLOGY,2012,118(0):8-12.
APA	Qiu, Weihua,&Chen, Hongzhang.(2012).Enhanced the enzymatic hydrolysis efficiency of wheat straw after combined steam explosion and laccase pretreatment.BIORESOURCE TECHNOLOGY,118(0),8-12.
MLA	Qiu, Weihua,et al."Enhanced the enzymatic hydrolysis efficiency of wheat straw after combined steam explosion and laccase pretreatment".BIORESOURCE TECHNOLOGY 118.0(2012):8-12.