Microcosmic Mechanisms for Protein Incomplete Release and Stability of Various Amphiphilic mPEG-PLA Microspheres

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	Microcosmic Mechanisms for Protein Incomplete Release and Stability of Various Amphiphilic mPEG-PLA Microspheres
	Wei, Yi 1,2; Wang, Yu Xia 1; Wang, Wei 3; Ho, Sa V.3; Qi, Feng 1,2; Ma, Guang Hui 1; Su, Zhi Guo 1
刊名	LANGMUIR
	2012-10-02
卷号	28 期号:39 页码:13984-13992
关键词	HUMAN GROWTH-HORMONE POLY-DL-LACTIDE-POLY(ETHYLENE GLYCOL) MICROSPHERES QUARTZ-CRYSTAL MICROBALANCE SUSTAINED-RELEASE MEMBRANE EMULSIFICATION PROCESS PARAMETERS DELIVERY-SYSTEM BIOMATERIAL ACID) ENCAPSULATION
ISSN号	0743-7463
通讯作者	Wang, YX
英文摘要	The microcosmic mechanisms of protein (recombinant human growth hormone, rhGH) incomplete release and stability from amphiphilic poly(monomethoxypolyethylene glycol-co-D,L-lactide) (mPEG-PLA, PELA) microspheres were investigated. PELA with different hydrophilicities (PELA-1, PELA-2, and PELA-3) based on various ratios of mPEG to PLA were employed to prepare microspheres exhibiting a narrow size distribution using a combined double emulsion and premix membrane emulsification method. The morphology, rhGH encapsulation efficiency, in vitro release profile, and rhGH stability of PELA microspheres during the release were characterized and compared in detail. It was found that increasing amounts of PLA enhanced the encapsulation efficiency of PELA microspheres but reduced both the release rate of rhGH and its stability. Contact angle, atomic force microscope (AFM), and quartz crystal microbalance with dissipation (QCM-D) techniques were first combined to elucidate the microcosmic mechanism of incomplete release by measuring the hydrophilicity of the PELA film and its interaction with rhGH. In addition, the pH change within the microsphere microenvironment was monitored by confocal laser scanning microscopy (CLSM) employing a pH-sensitive dye, which clarified the stability of rhGH during the release. These results suggested that PELA hydrophilicity played an important role in rhGH incomplete release and stability. Thus, the selection of suitable hydrophilic polymers with adequate PEG lengths is critical in the preparation of optimum protein drug sustained release systems. This present work is a first report elucidating the microcosmic mechanisms responsible for rhGH stability and its interaction with the microspheres. Importantly, this research demonstrated the application of promising new experimental methods in investigating the interaction between biomaterials and biomacromolecules, thus opening up a range of exciting potential applications in the biomedical field including drug delivery and tissue regeneration.
WOS标题词	Science & Technology ; Physical Sciences ; Technology
类目[WOS]	Chemistry, Multidisciplinary ; Chemistry, Physical ; Materials Science, Multidisciplinary
研究领域[WOS]	Chemistry ; Materials Science
关键词[WOS]	HUMAN GROWTH-HORMONE ; POLY-DL-LACTIDE-POLY(ETHYLENE GLYCOL) MICROSPHERES ; QUARTZ-CRYSTAL MICROBALANCE ; SUSTAINED-RELEASE ; MEMBRANE EMULSIFICATION ; PROCESS PARAMETERS ; DELIVERY-SYSTEM ; BIOMATERIAL ; ACID) ; ENCAPSULATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000309431100029
公开日期	2013-10-11
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3190]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100190, Peoples R China 3.Pfizer Inc, BioTherapeut R&D, Chesterfield, MO 63017 USA
推荐引用方式 GB/T 7714	Wei, Yi,Wang, Yu Xia,Wang, Wei,et al. Microcosmic Mechanisms for Protein Incomplete Release and Stability of Various Amphiphilic mPEG-PLA Microspheres[J]. LANGMUIR,2012,28(39):13984-13992.
APA	Wei, Yi.,Wang, Yu Xia.,Wang, Wei.,Ho, Sa V..,Qi, Feng.,...&Su, Zhi Guo.(2012).Microcosmic Mechanisms for Protein Incomplete Release and Stability of Various Amphiphilic mPEG-PLA Microspheres.LANGMUIR,28(39),13984-13992.
MLA	Wei, Yi,et al."Microcosmic Mechanisms for Protein Incomplete Release and Stability of Various Amphiphilic mPEG-PLA Microspheres".LANGMUIR 28.39(2012):13984-13992.