Strain Screening, Fermentation, Separation, and Encapsulation for Production of Nattokinase Functional Food

CORC > 过程工程研究所 > 中国科学院过程工程研究所 > 湿法冶金清洁生产技术国家工程实验室

	Strain Screening, Fermentation, Separation, and Encapsulation for Production of Nattokinase Functional Food
	Wei, Xuetuan 1,2,3; Luo, Mingfang 4; Xie, Yuchun 1,2; Yang, Liangrong 1,2; Li, Haojian 1,2; Xu, Lin 1,2,3; Liu, Huizhou 1,2
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2012-12-01
卷号	168 期号:7 页码:1753-1764
关键词	Nattokinase Screening Solid-state fermentation Separation Enteric coating
ISSN号	0273-2289
通讯作者	Luo, MF
英文摘要	This study presents a novel and integrated preparation technology for nattokinase functional food, including strain screening, fermentation, separation, and encapsulation. To rapidly screen a nattokinase-productive strain, PCR-based screening method was combined with fibrinolytic activity-based method, and a high productive strain, Bacillus subtilis LSSE-22, was isolated from Chinese soybean paste. Reduction of poly-gamma-glutamic acid (gamma-PGA) concentration may contribute to separation of nattokinase and reduction of late-onset anaphylaxis risk. Chickpeas were confirmed as the favorable substrate for enhancement of nattokinase production and reduction of gamma-PGA yield. Using cracked chickpeas, the nattokinase activity reached 356.25 +/- 17.18 FU/g (dry weight), which is much higher than previous reports. To further reduce gamma-PGA concentration, ethanol fractional extraction and precipitation were applied for separation of nattokinase. By extraction with 50 % and precipitation with 75 % ethanol solution, 4,000.58 +/- 192.98 FU/g of nattokinase powders were obtained, and the activity recovery reached 89 +/- 1 %, while gamma-PGA recovery was reduced to 21 +/- 2 %. To improve the nattokinase stability at acidic pH condition, the nattokinase powders were encapsulated, and then coated with methacrylic acid-ethyl acrylate copolymer. After encapsulation, the nattokinase was protected from being denatured under various acid conditions, and pH-responsible controlled release at simulated intestinal fluid was realized.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	BACILLUS-AMYLOLIQUEFACIENS DC-4 ; SUBTILISIN DJ-4 ; GENE ; PURIFICATION ; EXPRESSION ; DELIVERY ; CLONING ; NATTO ; ACID
收录类别	SCI
语种	英语
WOS记录号	WOS:000312205000003
公开日期	2013-10-09
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/2953]
专题	过程工程研究所_湿法冶金清洁生产技术国家工程实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Microbiol, Technol Transfer Ctr, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Wei, Xuetuan,Luo, Mingfang,Xie, Yuchun,et al. Strain Screening, Fermentation, Separation, and Encapsulation for Production of Nattokinase Functional Food[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2012,168(7):1753-1764.
APA	Wei, Xuetuan.,Luo, Mingfang.,Xie, Yuchun.,Yang, Liangrong.,Li, Haojian.,...&Liu, Huizhou.(2012).Strain Screening, Fermentation, Separation, and Encapsulation for Production of Nattokinase Functional Food.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,168(7),1753-1764.
MLA	Wei, Xuetuan,et al."Strain Screening, Fermentation, Separation, and Encapsulation for Production of Nattokinase Functional Food".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 168.7(2012):1753-1764.