Optimization of mixotrophic medium components for biomass production and biochemical composition biosynthesis by Chlorella vulgaris using response surface methodology

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > OSSO国家重点实验室

	Optimization of mixotrophic medium components for biomass production and biochemical composition biosynthesis by Chlorella vulgaris using response surface methodology
	Kong WB(孔维宝)1,2,3; Hua SF(华绍烽)1 ; Cao H(曹海)3; Mu YW(慕钰文)3; Yang H(杨红)3; Song H(宋昊)1,2 ; Xia CG(夏春谷)1
刊名	Journal of the Taiwan Institute of Chemical Engineers
	2012
卷号	43 期号:3 页码:360-367
关键词	Biomass production Biochemical composition biosynthesis Response surface methodology Mixotrophic medium components Chlorella vulgaris
ISSN号	1876-1070
通讯作者	孔维宝 ; 夏春谷
英文摘要	The design of an optimum and mixotrophic medium for biomass production and biochemical composition biosynthesis by Chlorella vulgaris was attempted by using response surface methodology. Based on the Plackett–Burman design, glucose, MgSO₄·7H₂O, KNO₃ and NaCl were selected from the eleven factors as the most critical mixotrophic nutrients. Subsequently, the selected four factors were investigated by the Box–Behnken design. Results showed that the maximum biomass yield of 4.28 g/L was predicted when the concentrations of glucose, MgSO₄·7H₂O, KNO₃ and NaCl were 25 g/L, 1.33 g/L, 1.30 g/L and 3.02 g/L, respectively. Verification of the optimization showed that the biomass content of C. vulgaris could be enhanced from 0.24 g/L in photoautotrophic SEM and 1.89 g/L in unoptimized mixotrophic medium to 4.24 g/L giving 17.67-fold and 2.24-fold increase in biomass production, respectively. The biochemical composition of C. vulgaris cultured in optimized mixotrophic medium indicated that the biomass can be used as the raw material for biorefinery.
学科主题	物理化学与绿色催化
收录类别	SCI
资助信息	National Science Found for Distinguished Young Scholars of China (Grant No. 20625308);Research Fund for Young Teachers of Northwest Normal University (Grant No. NWNU-LKQN-10-30)
语种	英语
WOS记录号	WOS:000304228500006
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/3897]
专题	兰州化学物理研究所_OSSO国家重点实验室
作者单位	1.Chinese Acad Sci, Lanzhou Inst Chem Phys, State Key Lab Oxo Synth & Select Oxidat, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.NW Normal Univ, Coll Iife Sci, Lanzhou 730070, Peoples R China
推荐引用方式 GB/T 7714	Kong WB,Hua SF,Cao H,et al. Optimization of mixotrophic medium components for biomass production and biochemical composition biosynthesis by Chlorella vulgaris using response surface methodology[J]. Journal of the Taiwan Institute of Chemical Engineers,2012,43(3):360-367.
APA	Kong WB.,Hua SF.,Cao H.,Mu YW.,Yang H.,...&Xia CG.(2012).Optimization of mixotrophic medium components for biomass production and biochemical composition biosynthesis by Chlorella vulgaris using response surface methodology.Journal of the Taiwan Institute of Chemical Engineers,43(3),360-367.
MLA	Kong WB,et al."Optimization of mixotrophic medium components for biomass production and biochemical composition biosynthesis by Chlorella vulgaris using response surface methodology".Journal of the Taiwan Institute of Chemical Engineers 43.3(2012):360-367.