Multiple in situ Nucleic Acid Collections (MISNAC) From Deep-Sea Waters | |
Wei, Zhan-Fei1,2; Li, Wen-Li2; Li, Jun2; Chen, Jun2; Xin, Yong-Zhi2; He, Li-Sheng2; Wang, Yong2 | |
刊名 | FRONTIERS IN MARINE SCIENCE |
2020-02-18 | |
卷号 | 7页码:10 |
关键词 | in situ filtration DNA extraction cell lysis deep-sea microbial community |
DOI | 10.3389/fmars.2020.00081 |
英文摘要 | To obtain an accurate picture of microbial processes is essential for in-depth understanding of the dynamics of microbial communities and metabolic potentials. In this study, an apparatus for automatic multiple in situ nucleic acid collections (MISNAC) loaded and controlled by a lander was designed and applied for in situ microbial filtration, cell lysis and nucleic acid collection at different time points during three cruises in the South China Sea. The MISNAC apparatus completed 390 L in situ water filtration and collected a total of 1,176 ng DNA with six working units in four deployments at similar to 1,000 m depth. Microbial community structures in water samples obtained by the MISNAC and in situ microbial filtration and fixation (ISMIFF) apparatus, respectively, were compared by analyzing sequences of 16S rRNA amplicons. The result showed that these communities were largely consistent, regardless of the differences in the communities in the samples from different cruises and time points. In addition, the transcriptomes of the samples collected by the MISANC and a Niskin bottle dramatically differed in abundance and diversity of 16S rRNA gene reads, indicating that more in situ RNA molecules were preserved by the MISNAC, compared to the Niskin. The application of the MISNAC apparatus paved the way to time-series changes of deep-sea microorganisms and their response to various environmental factors. |
资助项目 | National Key Research and Development Program of China[2016YFC0302501] |
WOS关键词 | SAR202 BACTERIOPLANKTON ; COMMUNITY ; SEQUENCES ; ABUNDANCE ; MICROBES ; ZONE |
WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
语种 | 英语 |
出版者 | FRONTIERS MEDIA SA |
WOS记录号 | WOS:000514298400001 |
资助机构 | National Key Research and Development Program of China |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://ir.idsse.ac.cn/handle/183446/7483] |
专题 | 深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组 |
通讯作者 | Wang, Yong |
作者单位 | 1.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Peoples R China |
推荐引用方式 GB/T 7714 | Wei, Zhan-Fei,Li, Wen-Li,Li, Jun,et al. Multiple in situ Nucleic Acid Collections (MISNAC) From Deep-Sea Waters[J]. FRONTIERS IN MARINE SCIENCE,2020,7:10. |
APA | Wei, Zhan-Fei.,Li, Wen-Li.,Li, Jun.,Chen, Jun.,Xin, Yong-Zhi.,...&Wang, Yong.(2020).Multiple in situ Nucleic Acid Collections (MISNAC) From Deep-Sea Waters.FRONTIERS IN MARINE SCIENCE,7,10. |
MLA | Wei, Zhan-Fei,et al."Multiple in situ Nucleic Acid Collections (MISNAC) From Deep-Sea Waters".FRONTIERS IN MARINE SCIENCE 7(2020):10. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论