Thermococcus aciditolerans sp. nov., a piezotolerant, hyperthermophilic archaeon isolated from a deep- sea hydrothermal vent chimney in the Southwest Indian Ridge

doi:10.1099/ijsem.0.004934

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 深海微生物细胞生物学研究组

	Thermococcus aciditolerans sp. nov., a piezotolerant, hyperthermophilic archaeon isolated from a deep- sea hydrothermal vent chimney in the Southwest Indian Ridge
	Li, Xue-Gong 5,6,7; Tang, Hong-Zhi 4,7; Zhang, Wei-Jia 5,6,7; Qi, Xiao-Qing 5,6,7; Qu, Zhi-Guo 3; Xu, Jun 1; Wu, Long-Fei 2
刊名	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
	2021
卷号	71 期号:8 页码:7
关键词	deep-sea hydrothermal vent Southwest Indian Ridge Thermococcus
ISSN号	1466-5026
DOI	10.1099/ijsem.0.004934
英文摘要	A hyperthermophilic, strictly anaerobic archaeon, designated strain SY113(T), was isolated from a deep-sea hydrothermal vent chimney on the Southwest Indian Ridge at a water depth of 2770 m. Enrichment and isolation of strain SY113(T) were performed at 85 degrees C at 0.1 MPa. Cells of strain SY113(T) were irregular motile cocci with peritrichous flagella and generally 0.8-2.4 mu m in diameter. Growth was observed at temperatures between 50 and 90 degrees C (optimum at 85 degrees C) and under hydrostatic pressures of 0.1-60 MPa (optimum, 27 MPa). Cells of SY113(T) grew at pH 4.0-9.0 (optimum, pH 5.5) and a NaCl concentration of 0.5-5.5 % (w/v; optimum concentration, 3.0 % NaCl). Strain SY113(T) was an anaerobic chemoorganoheterotroph and grew on complex proteinaceous substrates such as yeast extract and tryptone, as well as on maltose and starch. Elemental sulphur stimulated growth, but not obligatory for its growth. The G+C content of the genomic DNA was 55.0 mol%. Phylogenetic analysis of the 16S rRNA sequence of strain SY113(T) showed that the novel isolate belonged to the genus Thermococcus. On the basis of physiological characteristics, average nucleotide identity values and in silico DNA-DNA hybridization results, we propose a novel species, named Thermococcus aciditolerans sp. nov. The type strain is SY113(T) (=MCCC 1K04190(T)=JCM 39083(T)).
资助项目	National Key R and D Program of China[2018YFC0309904] ; National Key R and D Program of China[2016YFC0302502] ; National Natural Science Foundation of China[NSFC 91751202] ; National Natural Science Foundation of China[41806174] ; National Natural Science Foundation of China[91751108] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA19060403] ; Sanya City[2018YD01] ; Sanya City[2018YD02] ; Sanya City[2019YD16] ; Sanya City[2019PT38] ; Research and Development Project of Scientific Research Instruments and Equipment of Chinese Academy of Sciences[YJKYYQ20190053] ; Institute of Deep-sea Technology Innovation, Chinese Academy of Sciences[Y950071] ; Institute of Deep-sea Technology Innovation, Chinese Academy of Sciences[Y9719105] ; Centre National de la Recherche Scientifique
WOS关键词	PHOTOBACTERIUM-PROFUNDUM SS9 ; HYDROSTATIC-PRESSURE ; TEMPERATURE ; GROWTH ; BIOSYNTHESIS ; GUAYMASENSIS ; GENETICS
WOS研究方向	Microbiology
语种	英语
出版者	MICROBIOLOGY SOC
WOS记录号	WOS:000695241800023
资助机构	National Key R and D Program of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Sanya City ; Research and Development Project of Scientific Research Instruments and Equipment of Chinese Academy of Sciences ; Institute of Deep-sea Technology Innovation, Chinese Academy of Sciences ; Centre National de la Recherche Scientifique
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/8895]
专题	深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组
通讯作者	Li, Xue-Gong
作者单位	1.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai, Peoples R China 2.Aix Marseille Univ, CNRS, LCB, IMM,IM2B, Marseille, France 3.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Lab Deep Sea Informat Technol, Sanya, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Deep Sea Sci & Engn, CAS Key Lab Expt Study Deep Sea Extreme Condit, Sanya, Peoples R China 6.CNRS Marseille CAS Sanya, Int Associated Lab Evolut & Dev Magnetotact Multi, Sanya, Peoples R China 7.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Lab Deep Sea Microbial Cell Biol, Sanya, Peoples R China
推荐引用方式 GB/T 7714	Li, Xue-Gong,Tang, Hong-Zhi,Zhang, Wei-Jia,et al. Thermococcus aciditolerans sp. nov., a piezotolerant, hyperthermophilic archaeon isolated from a deep- sea hydrothermal vent chimney in the Southwest Indian Ridge[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2021,71(8):7.
APA	Li, Xue-Gong.,Tang, Hong-Zhi.,Zhang, Wei-Jia.,Qi, Xiao-Qing.,Qu, Zhi-Guo.,...&Wu, Long-Fei.(2021).Thermococcus aciditolerans sp. nov., a piezotolerant, hyperthermophilic archaeon isolated from a deep- sea hydrothermal vent chimney in the Southwest Indian Ridge.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,71(8),7.
MLA	Li, Xue-Gong,et al."Thermococcus aciditolerans sp. nov., a piezotolerant, hyperthermophilic archaeon isolated from a deep- sea hydrothermal vent chimney in the Southwest Indian Ridge".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 71.8(2021):7.