Application of L-Threonine Aldolase to on-DNA Reactions

doi:10.1021/acs.bioconjchem.1c00363

	Application of L-Threonine Aldolase to on-DNA Reactions
	Chai, Jing 1; Lu, Xiaojie 1,2,3; Arico-Muendel, Christopher C.1; Ding, Yun 1; Pollastri, Michael P.4
刊名	BIOCONJUGATE CHEMISTRY
	2021-09-15
卷号	32 期号:9 页码:1973-1978
ISSN号	1043-1802
DOI	10.1021/acs.bioconjchem.1c00363
通讯作者	Chai, Jing(jing.5.chai@gsk.com)
英文摘要	Enzymatic catalysis is a highly attractive approach to the DNA encoded library technology (DEL) that has not been widely explored. In this paper, we report an L-threonine aldolase (L-TA)-catalyzed on-DNA aldol reaction to form beta-hydroxy-alpha-amino acids, and its diastereoselectivity determination. L-TAs from three species show good on-DNA aldehyde scope and complementary stereoselectivity. The formed aldol product can be further diversified via various reactions, which demonstrates the utility of this reaction in DEL.
WOS关键词	ENCODED CHEMICAL LIBRARIES ; DRUG DISCOVERY ; SUBSTRATE ; SPECIFICITY ; TECHNOLOGY ; SELECTION ; ACIDS ; TOOL
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000697280800005
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/297884]
专题	中国科学院上海药物研究所
通讯作者	Chai, Jing
作者单位	1.GlaxoSmithKline, R&D Med Sci & Technol, Encoded Lib Technol NCE Mol Discovery, Cambridge, MA 02140 USA 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Northeastern Univ, Dept Chem & Chem Biol, Boston, MA 02115 USA
推荐引用方式 GB/T 7714	Chai, Jing,Lu, Xiaojie,Arico-Muendel, Christopher C.,et al. Application of L-Threonine Aldolase to on-DNA Reactions[J]. BIOCONJUGATE CHEMISTRY,2021,32(9):1973-1978.
APA	Chai, Jing,Lu, Xiaojie,Arico-Muendel, Christopher C.,Ding, Yun,&Pollastri, Michael P..(2021).Application of L-Threonine Aldolase to on-DNA Reactions.BIOCONJUGATE CHEMISTRY,32(9),1973-1978.
MLA	Chai, Jing,et al."Application of L-Threonine Aldolase to on-DNA Reactions".BIOCONJUGATE CHEMISTRY 32.9(2021):1973-1978.